Drug toxicity is one of the major reasons for drug attrition. Existing traditional methods do not have adequate in vitro predictability. Porsolt combines the most predictive cell models (e.g. primary cultures of target organs) with optimized assays specific to each type of toxicity, analysis and specific methods. We have accurate toxicity prediction in vitro services conducted in true target cells and within the physiological environment found with primary cultures.
Cardiotoxicity describes the property of a substance that damages the heart muscle and/or tissue.
Comprehensive in vitro Proarrhythmia Assay (CiPA):
|Electrophysiology measurement (conventional manual patch-clamp)||Cardiac ion channels|
|Proarrhythmic risk assessment (MEA & Calcium transient assay)||human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)|
Drug-induced vascular injury (DIVI) in pre-clinical toxicology studies involves damage to endothelial and smooth muscle cells.
|Coagulation impairment Tissue Factor and Thrombomodulin||HUVEC|
|Leucocyte recruitment VCAM-1, E-Selectin and ICAM-1||HUVEC|
Gastrointestinal system side effects are among the most common classes of adverse reactions associated with orally absorbed drugs.
|Gastric mucosal cell damage||Primary Rat gastric mucosal cells|
General Toxicity includes acute and preliminary chronic toxicity tests to help define and characterize the intrinsic toxicity of new substances.
|Acute toxicity||Rat – Mouse – Dog – Mini-pig|
|Preliminary chronic toxicity||Rat – Mouse|
Hepatotoxicity is the injury or damage to the liver caused by exposure to a substance.
|Cholestasis & bile canaliculi network||Primary hepatocytes (R) sandwich configuration|
|Cytolysis (2D & 3D)||Primary hepatocytes (H,R & M) and HepG2|
|Oxidative stress: Glutathione (GSH) depletion||Primary hepatocytes (H & R) and HepG2|
|Phospholipidosis||Primary hepatocytes (H & R) and HepG2|
|Steatosis: intracellular lipid accumulation triglycerides||Primary hepatocytes (H & R) and HepG2|
Nephrotoxicity is the deterioration or damage to the kidney function due to toxic effect of a substance.
|Cytolysis||Primary human renal proximal tubule epithelial cells Human renal proximal tubule epithelial cell line (HK-2) MDCK-II, CRFK,…|
|Lysosomal activity||Primary human renal proximal tubule epithelial cells Human renal proximal tubule epithelial cell line (HK-2)|
|Mitochondrial membrane potential||Primary human renal proximal tubule epithelial cells Human renal proximal tubule epithelial cell line (HK-2)|
Neurotoxicity is the effect caused to the normal activity of the nervous system by a substance.
|Cytolysis||Primary neurons (R,M) cell lines|
|Excitotoxicity Calcium measurement||Primary neurons (R,M) cell lines|
|Mitochondrial membrane potential||Primary neurons (R,M) cell lines|
|Neurite outgrowth||Primary neurons (R,M) cell lines|
Skin toxicity is the is the damage or deterioration of skin tissue after exposure to a substance.
|Cytotoxicity - Cell viability||3T3 & L929 fibroblasts|
|Ocular irritation (HET-CAM)||Chicken egg|
|Skin irritation||Reconstituted human epidermis|
|Skin sensitization||Monocyte cell line (THP1)|