INFLAMMATION
Inflammation is a response to a variety of stimuli, including damaged cells, irritants and pathogens, and is relevant for many different indications. Porsolt’s varied capabilities and expertise, together with multiple in vitro and in vivo models used for screening, efficacy, and safety assessment of potential compounds, allows us to address the specific needs of the industry.
SCROLL
INFLAMMATORY DISEASE
Immune
Response
An immune response is a reaction as a result of the introduction of a foreign substance.
IN VITRO MODELS
| Caspase 1 activation | Multiple cellular models |
| Caspase 3/7 activation | Multiple cellular models |
| Cell migration (Transwell chamber-based) | Multiple cellular models |
| Cell proliferation | Multiple cellular models |
| Cytolysis | Multiple cellular models |
| Immune cell activation and proliferation | Primary mouse splenocytes |
| Immune cell killing assay | Human T lymphocyte and tumor cells |
| Immune check point inhibitor | (PD1) - (PDL1) biochemical assay (HTRF) |
| Immune check point inhibitor | (CTLA-4) - (B7-1) biochemical assay (HTRF) |
| Immune T-cell infiltrationand killing assay (cytometry) | Multiple cellular models 3D co- culture |
| Lipid peroxidation | Multiple cellular models |
| Mitochondrial membrane potential | Multiple cellular models |
| Phagocytosis | Mouse – Rat Human macrophages |
| Sensitization | Monocytes (THP-1 cell line) |
In Vivo
Models
Inflammation caused by the introduction or insult with a foreign substance.
IN VIVO MODELS
| 12-tetradecanoylphorbol -13-acetate (TPA) - induced ear edema | Mouse |
| Air pouch | Mouse |
| Arachidonic acid-induced ear edema | Mouse |
| Carrageenan-induced abscess | Rat |
| Carrageenan-induced edema evaluation | Mouse - Rat |
| Lipopolysaccharide (LPS) Lung Injury (acute) | Mouse |
| Lipopolysaccharide (LPS)-induced neutrophilia | Ferret |
| Lipopolysaccharide (LPS)-induced septic shock | Mouse |
| Yeast-induced hyperthermia | Mouse |
BIOMARKER ASSAYS
| PLATFORM | BIOMARKER | SPECIES (SAMPLE) |
|---|---|---|
| Immunophenotyping (flow cytometry) | Leukocytes (Treg,Tc), neutrophils, monocytes, macrophages | Mouse, Human (blood, bloodbags, buffy coat, bone marrow, tumors…) |
| Histology (IHC-P) | Immune cell detection (CD8, CD3, CD68, CD45,…) | Rat, Mouse, multiple organs |
| CBA | Inflammation pathway IL-6, IL-10, MCP-1or IL-8 IFN-γ or IL-1β ,TNFα and IL-12p70 | Human (cell supernatant) |
| Flow cytometry (ICC) | STAT pathway (STAT1, STAT2, STAT3, STAT6…) | Human (blood) |
| Cell signaling (Erk, NFkB, C-jun) | Human (blood) | |
| ELISA | Inflammatory cytokines (TNFα ,IL-1β ,IL-6,IL-10) | Mouse (air pouch exsudat, plasma) |
| Inflammatory protein (CRP) | Rabbit (plasma, serum) | |
| Hematology | WBC (White Blood Cell count): neutrophils, macrophages, lymphocytes, eosinophils, basophils | Mouse (air pouch exsudat, plasma) |
| CBA - ELISA - WB - IHC-PIHC-Fr - ICC - HTRF | Other targets upon request | Mouse (air pouch exsudat, plasma) |
