INFLAMMATION

Inflammation is a response to a variety of stimuli, including damaged cells, irritants and pathogens, and is relevant for many different indications. Porsolt’s varied capabilities and expertise, together with multiple in vitro and in vivo models used for screening, efficacy, and safety assessment of potential compounds, allows us to address the specific needs of the industry.

SCROLL

Cytokins

INFLAMMATORY DISEASE

Immune
Response

An immune response is a reaction as a result of the introduction of a foreign substance.

IN VITRO MODELS
Caspase 1 activation Multiple cellular models
Caspase 3/7 activation Multiple cellular models
Cell migration (Transwell chamber-based) Multiple cellular models
Cell proliferation Multiple cellular models
Cytolysis Multiple cellular models
Immune cell activation and proliferation Primary mouse splenocytes
Immune cell killing assay Human T lymphocyte and tumor cells
Immune check point inhibitor (PD1) - (PDL1) biochemical assay (HTRF)
Immune check point inhibitor (CTLA-4) - (B7-1) biochemical assay (HTRF)
Immune T-cell infiltrationand killing assay (cytometry) Multiple cellular models 3D co- culture
Lipid peroxidation Multiple cellular models
Mitochondrial membrane potential Multiple cellular models
Phagocytosis Mouse – Rat Human macrophages
Sensitization Monocytes (THP-1 cell line)

In Vivo
Models

Inflammation caused by the introduction or insult with a foreign substance.

IN VIVO MODELS
12-tetradecanoylphorbol -13-acetate (TPA) - induced ear edema Mouse
Air pouch Mouse
Arachidonic acid-induced ear edema Mouse
Carrageenan-induced abscess Rat
Carrageenan-induced edema evaluation Mouse - Rat
Lipopolysaccharide (LPS) Lung Injury (acute) Mouse
Lipopolysaccharide (LPS)-induced neutrophilia Ferret
Lipopolysaccharide (LPS)-induced septic shock Mouse
Yeast-induced hyperthermia Mouse
BIOMARKER ASSAYS
PLATFORM BIOMARKER SPECIES (SAMPLE)
Immunophenotyping (flow cytometry) Leukocytes (Treg,Tc), neutrophils, monocytes, macrophages Mouse, Human (blood, bloodbags, buffy coat, bone marrow, tumors…)
Histology (IHC-P) Immune cell detection (CD8, CD3, CD68, CD45,…) Rat, Mouse, multiple organs
CBA Inflammation pathway IL-6, IL-10, MCP-1or IL-8 IFN-γ or IL-1β ,TNFα and IL-12p70 Human (cell supernatant)
Flow cytometry (ICC) STAT pathway (STAT1, STAT2, STAT3, STAT6…) Human (blood)
Cell signaling (Erk, NFkB, C-jun) Human (blood)
ELISA Inflammatory cytokines (TNFα ,IL-1β ,IL-6,IL-10) Mouse (air pouch exsudat, plasma)
Inflammatory protein (CRP) Rabbit (plasma, serum)
Hematology WBC (White Blood Cell count): neutrophils, macrophages, lymphocytes, eosinophils, basophils Mouse (air pouch exsudat, plasma)
CBA - ELISA - WB - IHC-PIHC-Fr - ICC - HTRF Other targets upon request Mouse (air pouch exsudat, plasma)

Porsolt

Z.A. de Glatigné
53940 Le Genest-Saint-Isle, France
Telephone +33 2 43 69 36 07

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