About Us
Porsolt is an internationally recognized and long established global preclinical Contract Research Organization (CRO) long, that has been providing efficacy evaluation and safety pharmacology services for over 35 years. Porsolt’s capabilities and services cover the drug development process from early screening through regulatory submission, including comprehensive safety evaluation of drug candidates in full compliance with GLP.
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About Porsolt
Porsolt was first audited for GLP Compliance in the year 2000, and has continuously maintained this status, with continued successful bi-annual inspections by the independent ANSM authority. Porsolt is also an AAALAC Accredited facility.
The quality of our work can be traced to the expert qualifications of our staff, with more than 150 years of combined scientific experience. This, together with over 35 years of institutional memory, provides a valuable source of advice and guidance for our clients with their development programs.
Porsolt’s clients include major Pharmaceutical Companies, Biotechnology Companies, Foundations, Not-For-Profits and Academic Institutions, from all around the world, with hundreds of studies being performed annually.
We aim to be an extension of our client’s team, effectively supporting R&D programs with validated and innovative solutions. We combine our core values of personalized customer service, flexibility, and scientific innovation, to provide a consistently superior service.
Capabilities
Biomarker Assays
| Calcium (total) |
| Magnesium, Phosphorus, Sodium, Potassium |
| Chloride/Triglycerides/Creatinine |
| Total Bilirubin |
| AST (Aspartate Aminotransferase)/ALP (Alkaline Phosphatase)/ALT (Alanine Aminotransferase) |
| GGT (Gamma Glutamyl Transferase) |
| Cholesterol/HDL cholesterol/LDL cholesterol/Glucose |
| NEFA (Non Esterified Fatty Acids) |
| Total proteins/Urea/Albumin |
| Amylase (pancreatic)/Lipase |
| Insulin/Glucagon |
| Adiponectin/Leptin |
| Creatin |
| Sodium, Potassium, Chloride |
| Albumin (microalbumin)/Total proteins/Semi-quantitative parameters |
| LDH (lactate deshydrogenase) |
| HbA1c (glycated hemoglobin) |
| APTT (Activated Partial Thrombin Time) |
| Prothrombin Time/Fibrinogen |
| Complete blood count including red blood cell, hemoglobin, hematocrit, mean cell volume, mean cell, hemoglobin, mean cell hemoglobin concentration, platelets, white blood cell, neutrophils, lymphocytes, monocytes, eosinophils, basophils. |
Imuno Assays
- ELISA
- HTRF (Homogeneous Time
Resolved Fluorescence)
- Immunostaining of cell surface
and intracellular biomarkers
- Multiplexed immunophenotyping
& biomarker detection by automated
3-laser flow cytometry
- Multiplexed analysis of soluble and intracellular
protein expression by the BD™
Cytometric Bead Array (CBA) technique
- High-throughput flow cytometry
- FLIPR Tetra high
-throughput cellular
screening system
- High-content histology:
- tissue sections
- 3D microtissues
- tissue microarray,…
- High-throughput image acquisition
of IF/IHC-stained tissue sections
or tissue microarrays
Cell & Tissue Banks
Fresh blood from healthy donors through an agreement with the French blood bank.
Primary cell cultures:
- Cryopreserved primary cells (rodents and human)
- Freshly isolated rodent primary cell cultures:
- neurons, astrocytes, oligodendrocytes, microglia
- pituitary cells
- hepatocytes
- splenocytes
Human Induced Pluripotent Stem Cells (hIPSC)
derived cells:
- hiPSC derived
dopaminergic neurons
- iCell2® (Cardiomyocytes)
Cell lines:
- Cancer cell lines: leukemia, solid tumors
- Normal immortalized cell lines
- Cell lines expressing our proprietary
specific biosensors
Artificial 3D microtissues
Human & animal tissue access & processing
(commercial and tailor-made
Tissues Micro Arrays)
Formulation Analysis
Porsolt’s vast portfolio of models and services for different indications also allows us to provide a comprehensive follow-up analysis of your project as part of a larger development program.
Method transfer or development
Method validation:
- specificity - accuracy
- linearity - precision,…
Stability evaluation
(stock and dosing formulations)
Adsorption evaluation
(for in vitro studies)
Formulation analysis:
- concentration verification
- homogeneity of formulations
Image Processing & Data Analysis
Image processing and data analysis provide very useful tools for biological applications. Firstly, acquired images are described based on adapted and customized features (texture, color, grey-level, shape, etc.) The obtained image information is classified using data analysis and visualization methods.
Image acquisition:
- High image resolution
- Tissue preview and ROI selection
- Brightfield and fluorescence for IHC and IF
Quantitative image analysis:
- Intensity of labeling
- Subcellular target characterization
- Event counting
- ROI detection
- Tissue structure description
(shape, tissular organization, etc.)
- Features linked to neuronal biology
- Indices to assist pathologists with
their diagnoses (e.g. in oncology)
Clients can send their own images
(from their own experiments)
for quantitative image & data analysis and pathology
review
Technical Skill
Brain/ Neurosurgery
Cystometry
Induction of cognitive deficits
Induction of neuropathic pain
Ligation models
Medical device implantation
Nephrectomy
Post-operative pain
Pump administration
Post-operative pain
Pump administration
Technical Capabilities
Equipments
Conventional animal facility | IVC housing | Biosafety level 2 (BSL2) facilities
Facilities
HPLC
High-throughput
Fluorometric
Luminometric Imaging
Plate Reader (FLIPR)
Automated multipurpose
plate reader (e.g. for HTRF,
Absorbance, Luminescence,
Fluorescence measurements)
High-content imaging
Immunohistology
processing platform
Live-content imaging
Automated flow cytometry
Automated video detection
In vivo telemetry systems
High-throughput label-free
and image-based cytometry
Biochemistry analyzer
Hematology analyzer
Non-invasive multimodal in vivo
imaging (e.g. for luminescence,
Absorbance, and X-ray)
Ultrasound imaging
