DERMATOLOGY
Dermatological diseases such as Psoriasis, Allergic Contact Dermatitis (ACD) and Atopical Dermatitis (AD or Irritant Contact Dermatitis) are major skin diseases of immunological origin, and represent a major health problem due to the substantial patient population that is affected.
Porsolt offers in vitro and in vivo models that allow for testing at multiple stages of the drug development process.
SCROLL
DERMATOLOGY
IN VITRO MODELS
IN VIVO MODELS
INFLAMMATORY PATHWAY AND ANTI-INFLAMMATORY ACTIVITY
| Atopic Dermatitis - Poly (I:C) induced cytokine release | NHEK |
| Cannabinoid anti-inflammatory evaluation Cytokine release | NHEK |
| Cytokine release | Keratinocytes Dendritic cells (Langerhans) |
| IL-6 induced secretion (by IL-17) | NHDF |
| Immune cell activation | Lymphocytes, PBMC Dendritic cells (Langerhans) |
| Immune cell proliferation | Lymphocytes, PBMC |
| Immune cells phagocytosis (E.coli particles) | Macrophages Dendritic cells (Langerhans) |
| TNFα induced cytotoxicity | L929 |
OXIDATIVE DAMAGE AND ANTI-OXIDANT POTENTIAL
| Atopic Dermatitis - Poly (I:C) induced cytokine release | NHEK |
| Cannabinoid anti-inflammatory evaluation Cytokine release | NHEK |
| Cytokine release | Keratinocytes Dendritic cells (Langerhans) |
| IL-6 induced secretion (by IL-17) | NHDF |
| Immune cell activation | Lymphocytes, PBMC Dendritic cells (Langerhans) |
| Immune cell proliferation | Lymphocytes, PBMC |
| Immune cells phagocytosis (E.coli particles) | Macrophages Dendritic cells (Langerhans) |
| TNFα induced cytotoxicity | L929 |
PIGMENTATION
| Endothelin signaling pathway - Calcium | Normal human epidermal melanocyte (NHEM) |
PREDICTIVE TOXICITY
| Cytotoxicity - Cell viability | Cell lines (3T3, L929, HaCaT) NHEK, NHDF |
| Ocular irritation HET-CAM | Chicken egg |
| Skin irritation | Reconstituted human epidermis |
| Skin irritation | Monocyte cell line (THP1) |
PROTECTION AGAINST POLLUTION
| Indoor dust - Inflammatory cytokine release | Dendritic cells (Langerhans) |
| Urban dust - Inflammatory cytokine release | NHEK Dendritic cells (Langerhans) |
| Urban dust - Lipid peroxidation | NHEK |
| Urban dust - Reactive Oxygen Species induction (ROS) | NHEK |
SKIN AGING
| Senescence (oxidative stress induction or high passage senescence) | Keratinocytes |
| Total collagen secretion | Elderly fibroblast donor |
| Wound healing | NHEK |
| Elderly fibroblast or keratinocyte donor | NHEK |
SKIN MICROCIRCULATION
| Angiogenesis | NHDF & HUVEC |
| Calcitonin Gene Related Peptide signaling pathways (CGRP) | HDMEC |
SKIN REGENERATION
| Cell migration/Wound healing | HaCaT, NHEK, NHDF |
| Cell proliferation | HaCaT, NHEK, NHDF |
| Total collagen formation | NHDF |
IN VIVO
| Allergic Contact Dermatitis | Pig |
| Imiquimod-induced psoriasis-like skin inflammation | Mouse |
| Pruritogens-induced scratching behavior | Mouse – Rat |
| Wound healing | Mouse |
BIOMARKER ASSAYS
| PLATFORM | BIOMARKER | SPECIES (SAMPLE) |
|---|---|---|
| Histology (IHC-P) | Skin structure (keratin10, loricrin, filaggrin, elastin, involucrin, e-cadherin, ZO-1) | Human (explant or RHE) |
| Cell proliferation (Ki67,…) | Human (explant or RHE) | |
| Immune cells (macrophages, T-cells, Langerhans,…) | Human (explant or RHE), Mouse, Rat | |
| Staining (paraffin slices) | Hematoxylin & Eosin, Sirius red, Masson’s trichrome,… | Multiple species & tissues |
| CBA | Inflammation pathway IL-6, IL-10, MCP-1or IL-8 IFN-γ or IL-1β ,TNF and IL-12p70 | Human (cell supernatant) |
| ELISA | Inflammatory cytokine (TNF-α , IL-6, IL-17) | Mouse (ear) |
| CBA - ELISA - WB - IHC-PIHC-Fr - ICC - HTRF | Other targets upon request | Multiple species & biological specimens |
