ONCOLOGY

Oncology is an area that commands a larger proportion of the research world’s resources.
Porsolt is now able to provide services using its Oncology in vitro capabilities and expertise.

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Oncology

ONCOLOGY DISEASE

Cell Stress, DNA Damage and Metabolism

Cell Stress, DNA Damage and Metabolism

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The reaction of cells to a wide different types of stressors, including cancers infiltration, infection, extremes of temperature, toxins, and mechanical damage.

BIOCHEM.
Cell metabolism (DiHydroFolate Reductase DHFR activity) Recombinant enzyme
IN VITRO MODELS
Apoptosis assay(Caspase 3/7 activation) Multiple cellular models
Autophagy assay(LC3B turnover assay) HeLa cells
Cell metabolism(Neutral lipids intracellular accumulation) Multiple cellular models
Cell metabolism(Phospholipids intracellular accumulation) Multiple cellular models
Cell metabolism (Protein synthesis quantification) Multiple cellular models
Cell metabolism(Protein synthesis quantification) Multiple cellular models
Cell stress assay (intracellular reactive oxygen species ROS) Multiple cellular models
Cell stress assay (Lipid peroxidation) Multiple cellular models
Cell stress assay (Mitochondrial membrane potential) Multiple cellular models
Hypoxia assay (HIF1α quantification) Multiple cellular models
Hypoxia assay (Pymonidazole metabolite quantification) Multiple cellular models

Immuno-oncology

Immuno-oncology focuses on stimulation of the immune system to treat cancer.

BIOCHEM.
Binding assay of immune check points inhibitors Multiple inhibitors (HTRF)
IN VITRO MODELS
Immune T-cell infiltration and killing assay cytometry 3D co-culture multiple cells
Immune T-cell killing assay live-content imaging 2D co-culture multiple cells
T-cell activation assay live-content imaging Human peripheral mononuclear blood cell (hPBMC/CD3 cells)
IN VIVO MODELS
Colon cancer (anti-PD-1/CTLA-4) CT26.WT cells Mouse
Glioblastoma (anti-PD-1/CTLA-4) GL261 cells Mouse
Renal cancer (anti-PD-1/CTLA-4) RenCa cells Mouse

Receptor Pharmacology & Signaling Pathways

Receptor Pharmacology & Signaling Pathways

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Focusing on the receptors and pathways involved in controling the cancer.

IN VITRO MODELS
AKT phosphorylation Multiple cellular models
Androgen receptor nuclear translocation LNCaP cell line
Calcium homeostasis Multiple cellular models
cAMP quantification Multiple cellular models
ERK activation (pERK1/2) Multiple cellular models
NFkB activation Multiple cellular models
Prostate Specific Antigen (PSA) expression LNCap cell line

Targeting Angiogenis

Targeting the blood supply associated with the cancer.

IN OVO MODELS
Assay (screening – 3R approach) Chicken eggs
IN VITRO MODELS
Tube formation assay live-content imaging HUVECs cells co-cultured with fibroblasts

Targeting Metastasis

Metastasis is the spread of cancer cells from the primary site of origin to new areas of the body.

IN OVO MODELS
Experimental metastasis CAM Assay (screening – 3R approach) Chicken eggs
IN VIVO MODELS
Experimental lung metastasis syngeneic model of breast cancer 4T1 cells
Experimental lung metastasis syngeneic model of colon cancer CT26.WT cells
Experimental lung metastasis xenograft model of breast cancer MDA-MB-231 cells Mouse

Targeting Tumor Cells

Targeting specific tumor cells as a way of combating the cancer.

IN OVO MODELS
Chicken Chorioallantoic Membrane (CAM) xenograft assay (screening – 3R approach) Multiple cellular models Chicken eggs
IN VITRO MODELS
Apoptosis assay Multiple 2D cellular models
Cell cycle cytometry analysis Multiple 2D or 3D cellular models
Cell proliferation/cytolysis assay high-content imaging Multiple 2D cellular models
Cell viability colorimetric assay Multiple 2D cellular models
Clonogenicity assay (anchorage-independent) Multiple 3D cellular models
Invasion assay Multiple 3D cellular models
Migration assay – high-content imaging Multiple 2D cellular models
Spheroid proliferation/cytolysis assay high-content imaging Multiple 3D cellular models
IN VIVO MODELS
Hollow fiber assay Multiple cellular models (screening – 3R approach) Mouse – Rat

Orthotopic syngeneic models of:

Breast cancer 4T1 cells – Mouse
Colon cancer including neurological and behavior analysis CT26.WT/C26 cells - Mouse
Glioblastoma (brain tumor) GL261 cells - Mouse
Kidney cancer RenCa cells - Mouse
Lung cancer including respiratory function LLC1/KLN205 cells - Mouse

Orthotopic xenograft models of:

Breast cancer MDA-MB -231 cells - Mouse
Lung cancer A549/PC-9 cells Mouse
Glioblastoma U87MG cells in mouse

Subcutaneous syngeneic model of:

Breast cancer 4T1 cells – Mouse
Colon cancer CT26.WT/C26 cells - Mouse
Glioblastoma (brain cancer) GL261 cells - Mouse
Lung cancer LLC1/KLN205 cells - Mouse
Renal Cancer 105K cells (Tuberous Sclerosis Alliance)

Subcutaneous xenograft models of:

Bladder cancer SW780 cells - Mouse
Breast cancer MDA-MB -231/BT-20 cells Mouse
Colon cancer HCT-8/HCT-116 cells Mouse
Fibrosarcoma HT-1080 cells - Mouse
Glioblastoma (brain tumor) U118MG/U87MG/U138MG cells Mouse
Kidney cancer ACHN cells - Mouse
Liver cancer Hep3B2.1-7/HepG2 cells Mouse
Lung cancer A549/PC-9 cells - Mouse
Pancreatic cancer PANC-1 cells - Mouse
Prostate cancer LNCaP/PC-3 - Mouse

Tumor-Associated Side Effects

Tumor-Associated Side Effects

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Side effects are secondary to the main intended effect of a treatment.

PAIN

IN VIVO MODELS
Chemotherapy-induced intestinal mucositis DRGs Rat
IN VITRO MODELS
Chemotherapy-induced intestinal mucositis Mouse
Chemotherapy - induced neuropathic pain: Vincristine model Rat

CACHEXIA

IN VIVO MODELS
Drug-induced cachexia model Rat
Tumor-induced cachexia model AH-130 cells (Rat)
Tumor-induced cachexia model C26 cells (Mouse)
Tumor-induced cachexia model LLC1 cells (Mouse)
BIOMARKER ASSAYS
PLATFORM BIOMARKER SPECIES (SAMPLE)
Immunophenotyping Regulatory T-cells (CD4, FoXP3) Cytotoxic T-cells (CD8) Mouse
Histology (IHC-P) Breast cancer (ER, PR, HER-2) Human PDX
Epithelial to mesenchymal transition (E-cadherin,Vimentin) Human PDX
Immune cells (T-cells, macrophages,…) Rat, Mouse
Signaling pathways (apoptosis, proliferation, hypoxia) Rat, Mouse
Staining (paraffin slices) Hematoxylin & Eosin, Sirius red, Masson’s trichrome,… Multiple species
CBA Th1/Th2/Th17 pathway (IL-2, IL-4, IL-6, IFN-γ , TNF α, IL-17A, IL-10) Mouse, Human
Inflammation pathway (IL-6, IL-10, MCP-1, IL-8, IFN-γ , IL-1β , TNF, IL-12p70) Mouse, Human
WB Ubiquitin, Lamin B1, ERK1/2, P-ERK1/2, P-S6k1, AKT, P-AKT, GSK3, P-GSK3, GAPDH Mouse, Rat (multiple cells and tissues)
Multimodal acquisition Cell metabolism (DHFR, protein synthesis), Cell stress (ROS, lipid peroxidation, intracellular phospholipid accumulation, mitochondrial membrane potential, GSH), Hypoxia (Pymonidazole), Autophagy (LC3B), Signaling (ERK1/2, NfKB, AKT, AR, PSA, cAMP, calcium homeostasis)
CBA - ELISA - WB - IHC-PIHC-Fr - ICC - HTRF Other targets upon request Multiple species & biological specimens

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Z.A. de Glatigné
53940 Le Genest-Saint-Isle, France
Telephone +33 2 43 69 36 07

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