ONCOLOGY
Oncology is an area that commands a larger proportion of the research world’s resources.
Porsolt is now able to provide services using its Oncology in vitro capabilities and expertise.
SCROLL
ONCOLOGY DISEASE
Cell Stress, DNA Damage and Metabolism
The reaction of cells to a wide different types of stressors, including cancers infiltration, infection, extremes of temperature, toxins, and mechanical damage.
BIOCHEM.
IN VITRO MODELS
| Cell metabolism (DiHydroFolate Reductase DHFR activity) | Recombinant enzyme |
| Apoptosis assay(Caspase 3/7 activation) | Multiple cellular models |
| Autophagy assay(LC3B turnover assay) | HeLa cells |
| Cell metabolism(Neutral lipids intracellular accumulation) | Multiple cellular models |
| Cell metabolism(Phospholipids intracellular accumulation) | Multiple cellular models |
| Cell metabolism (Protein synthesis quantification) | Multiple cellular models |
| Cell metabolism(Protein synthesis quantification) | Multiple cellular models |
| Cell stress assay (intracellular reactive oxygen species ROS) | Multiple cellular models |
| Cell stress assay (Lipid peroxidation) | Multiple cellular models |
| Cell stress assay (Mitochondrial membrane potential) | Multiple cellular models |
| Hypoxia assay (HIF1α quantification) | Multiple cellular models |
| Hypoxia assay (Pymonidazole metabolite quantification) | Multiple cellular models |
Immuno-oncology
Immuno-oncology focuses on stimulation of the immune system to treat cancer.
BIOCHEM.
IN VITRO MODELS
IN VIVO MODELS
| Binding assay of immune check points inhibitors | Multiple inhibitors (HTRF) |
| Immune T-cell infiltration and killing assay cytometry | 3D co-culture multiple cells |
| Immune T-cell killing assay live-content imaging | 2D co-culture multiple cells |
| T-cell activation assay live-content imaging | Human peripheral mononuclear blood cell (hPBMC/CD3 cells) |
| Colon cancer (anti-PD-1/CTLA-4) | CT26.WT cells Mouse |
| Glioblastoma (anti-PD-1/CTLA-4) | GL261 cells Mouse |
| Renal cancer (anti-PD-1/CTLA-4) | RenCa cells Mouse |
Receptor Pharmacology & Signaling Pathways
Focusing on the receptors and pathways involved in controling the cancer.
IN VITRO MODELS
| AKT phosphorylation | Multiple cellular models |
| Androgen receptor nuclear translocation | LNCaP cell line |
| Calcium homeostasis | Multiple cellular models |
| cAMP quantification | Multiple cellular models |
| ERK activation (pERK1/2) | Multiple cellular models |
| NFkB activation | Multiple cellular models |
| Prostate Specific Antigen (PSA) expression | LNCap cell line |
Targeting Angiogenis
Targeting the blood supply associated with the cancer.
IN OVO MODELS
IN VITRO MODELS
| Assay (screening – 3R approach) | Chicken eggs |
| Tube formation assay live-content imaging | HUVECs cells co-cultured with fibroblasts |
Targeting Metastasis
Metastasis is the spread of cancer cells from the primary site of origin to new areas of the body.
IN OVO MODELS
IN VIVO MODELS
| Experimental metastasis CAM Assay (screening – 3R approach) | Chicken eggs |
| Experimental lung metastasis syngeneic model of breast cancer | 4T1 cells |
| Experimental lung metastasis syngeneic model of colon cancer | CT26.WT cells |
| Experimental lung metastasis xenograft model of breast cancer | MDA-MB-231 cells Mouse |
Targeting Tumor Cells
Targeting specific tumor cells as a way of combating the cancer.
IN OVO MODELS
IN VITRO MODELS
IN VIVO MODELS
| Chicken Chorioallantoic Membrane (CAM) xenograft assay (screening – 3R approach) | Multiple cellular models Chicken eggs |
| Apoptosis assay | Multiple 2D cellular models |
| Cell cycle cytometry analysis | Multiple 2D or 3D cellular models |
| Cell proliferation/cytolysis assay high-content imaging | Multiple 2D cellular models |
| Cell viability colorimetric assay | Multiple 2D cellular models |
| Clonogenicity assay (anchorage-independent) | Multiple 3D cellular models |
| Invasion assay | Multiple 3D cellular models |
| Migration assay – high-content imaging | Multiple 2D cellular models |
| Spheroid proliferation/cytolysis assay high-content imaging | Multiple 3D cellular models |
| Hollow fiber assay Multiple cellular models (screening – 3R approach) | Mouse – Rat |
Orthotopic syngeneic models of:
| Breast cancer | 4T1 cells – Mouse |
| Colon cancer including neurological and behavior analysis | CT26.WT/C26 cells - Mouse |
| Glioblastoma (brain tumor) | GL261 cells - Mouse |
| Kidney cancer | RenCa cells - Mouse |
| Lung cancer including respiratory function | LLC1/KLN205 cells - Mouse |
Orthotopic xenograft models of:
| Breast cancer | MDA-MB -231 cells - Mouse |
| Lung cancer | A549/PC-9 cells Mouse |
| Glioblastoma | U87MG cells in mouse |
Subcutaneous syngeneic model of:
| Breast cancer | 4T1 cells – Mouse |
| Colon cancer | CT26.WT/C26 cells - Mouse |
| Glioblastoma (brain cancer) | GL261 cells - Mouse |
| Lung cancer | LLC1/KLN205 cells - Mouse |
| Renal Cancer | 105K cells (Tuberous Sclerosis Alliance) |
Subcutaneous xenograft models of:
| Bladder cancer | SW780 cells - Mouse |
| Breast cancer | MDA-MB -231/BT-20 cells Mouse |
| Colon cancer | HCT-8/HCT-116 cells Mouse |
| Fibrosarcoma | HT-1080 cells - Mouse |
| Glioblastoma (brain tumor) | U118MG/U87MG/U138MG cells Mouse |
| Kidney cancer | ACHN cells - Mouse |
| Liver cancer | Hep3B2.1-7/HepG2 cells Mouse |
| Lung cancer | A549/PC-9 cells - Mouse |
| Pancreatic cancer | PANC-1 cells - Mouse |
| Prostate cancer | LNCaP/PC-3 - Mouse |
Tumor-Associated Side Effects
Side effects are secondary to the main intended effect of a treatment.
PAIN
IN VIVO MODELS| Chemotherapy-induced intestinal mucositis | DRGs Rat |
| Chemotherapy-induced intestinal mucositis | Mouse |
| Chemotherapy - induced neuropathic pain: Vincristine model | Rat |
CACHEXIA
IN VIVO MODELS| Drug-induced cachexia model | Rat |
| Tumor-induced cachexia model | AH-130 cells (Rat) |
| Tumor-induced cachexia model | C26 cells (Mouse) |
| Tumor-induced cachexia model | LLC1 cells (Mouse) |
BIOMARKER ASSAYS
| PLATFORM | BIOMARKER | SPECIES (SAMPLE) |
|---|---|---|
| Immunophenotyping | Regulatory T-cells (CD4, FoXP3) Cytotoxic T-cells (CD8) | Mouse |
| Histology (IHC-P) | Breast cancer (ER, PR, HER-2) | Human PDX |
| Epithelial to mesenchymal transition (E-cadherin,Vimentin) | Human PDX | |
| Immune cells (T-cells, macrophages,…) | Rat, Mouse | |
| Signaling pathways (apoptosis, proliferation, hypoxia) | Rat, Mouse | |
| Staining (paraffin slices) | Hematoxylin & Eosin, Sirius red, Masson’s trichrome,… | Multiple species |
| CBA | Th1/Th2/Th17 pathway (IL-2, IL-4, IL-6, IFN-γ , TNF α, IL-17A, IL-10) | Mouse, Human |
| Inflammation pathway (IL-6, IL-10, MCP-1, IL-8, IFN-γ , IL-1β , TNF, IL-12p70) | Mouse, Human | |
| WB | Ubiquitin, Lamin B1, ERK1/2, P-ERK1/2, P-S6k1, AKT, P-AKT, GSK3, P-GSK3, GAPDH | Mouse, Rat (multiple cells and tissues) |
| Multimodal acquisition | Cell metabolism (DHFR, protein synthesis), Cell stress (ROS, lipid peroxidation, intracellular phospholipid accumulation, mitochondrial membrane potential, GSH), Hypoxia (Pymonidazole), Autophagy (LC3B), Signaling (ERK1/2, NfKB, AKT, AR, PSA, cAMP, calcium homeostasis) | |
| CBA - ELISA - WB - IHC-PIHC-Fr - ICC - HTRF | Other targets upon request | Multiple species & biological specimens |
