liver & hepatic system
Porsolt’s experience in preclinical safety and efficacy has led to the development of models that focus on the liver and hepatic system. This is an important part of the process to better understand the effects of compounds on the liver and hepatic system.
SCROLL
LIVER & HEPATIC SYSTEM
IN VITRO MODELS
IN VIVO MODELS
| Acetaminophen (acute model) | Mouse |
| Cholestasis/Bile canaliculi network | Primary hepatocytes sandwich configuration, Rat |
| Glutathione (GSH), intracellular GSH content | Primary human and rat hepatocytes |
| Steatosis/Lipid, intracellular accumulation: neutral lipids | Primary human and rat hepatocytes |
| Cytolysis | Primary human and rat hepatocytes |
| Lipid, intracellular accumulation: phospholipids | Primary human and rat hepatocytes |
| 3D Hepatotoxicity (Viability) | Primary human hepatocyte spheroids |
| Acetaminophen (acute model) | Mouse |
| Bile Duct Ligation (BDL) (chronic model) | Rat |
| Carbon tetrachloride (CCl4) (acute model) | Rat |
BIOMARKER ASSAYS
| PLATFORM | BIOMARKER | SPECIES (SAMPLE) |
|---|---|---|
| Histology (IHC-P) | Hepatic transporters (MRP-2, ZO-1,…) | Rat (liver) |
| Hepatocellular ballooning (H&E, p62) | Rat (liver) | |
| Staining (paraffin slices) | Hematoxylin & Eosin, Sirius red, Masson’s trichrome,… | Multiple species & tissues |
| Biochemical | Lipid panel (HDL, LDL, FFA, TG, cholesterol…) | Mouse, Rat (plasma, serum) |
| Liver function panel (ALAT, ASAT, ALP, bilirubin…) | Mouse, Rat (plasma, serum) | |
| ELISA | Inflammatory cytokines (TNF-α /IL-1β /IL-6) | Mouse, Rat (plasma, serum) |
| CBA - ELISA - WB - IHC-PIHC-Fr - ICC - HTRF | Other targets upon request | Multiple species & biological specimens |
| Signaling Pathways (IF in vitro) | Nrf2 pathway (Nrf2, HO-1 and NQO1) | Mouse, Human, Rat |
| ATP-binding cassette (ABC) transporters (MRP-2) | Mouse, Human, Rat |
