Combined high throughput screening and high content analysis approaches to neuronal physiologypathology monitoring provide powerful tools

Neuronal physiology is complex and monitoring this in vitro includes multiple potential measurements such as calcium homeostasis, cell membrane and mitochondrial membrane potential, survival, neurite network, protein expression and phosphorylation, and synaptogenesis. These parameters are dynamic, and can be measured over different time scales, requiring different technologies.  Typically, these measurements are considered separately for drug discovery, where miniaturization and quick turnaround are required. However, drug screening using only one parameter for primary molecule selection can yield simplistic results, when the most useful molecules already approved for CNS indications have complex profiles. In order to better map the effects of compounds, we used a combination of technological approaches to provide a continuum between early and late events of neuronal physiology.

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