IN VITRO CARDIOVASCULAR STUDIES

hERG channel test

Conventional manual patch-clamp assays (whole-cell configuration): hERG is the gold standard for ion channel investigations allowing sensitive and reliable analysis of the electrical activity of cell membranes.

- Experiment conducted at physiological temperature
- Experiment manually controlled by a highly-trained technician
- Varying durations of test substance incubation
- Analysis of the concentrations of the superfused solutions (HPLC)

Purkinje fiber

In vitro electrophysiology assays using multicellular preparations such as  cardiac Purkinje fiber or guinea pig papillary muscle complement patch-clamp studies and can provide valuable information about the effect of test substances on transmembrane action potentials.
Different ionic currents, including but not limited to the potassium current, contribute to the action potential shape and electrocardiogram wave form, suggesting that multicellular preparations can therefore provide additional information to aid the decision-making process when assessing novel substances for potential risks associated with cardiac liability.

in vitro follow-up studies

While the hERG channel is still considered to be the main target for in vitro proarrhythmic risk assessment, a rapid and preliminary test of the L type calcium current (ICaL), the rapid component of the sodium current (INa) and the ionic currents responsible for maintaining resting membrane potential (the inward rectifier potassium current IK1) using the patch-clamp technique in transfected cells is recommended for drawing a more complete cardiac risk profile of a new drug.

Our main supplemental studies:

> hERG trafficking

> hNav1.5, hCav1.2 and hKir2.1 channels

> Isolated vascular tissues

> Isolated atrium