NEUROTOXICITY

Neurotoxicity represents frequent and troublesome side effects, making the central and peripheral nervous systems important targets of toxicological studies. Fluofarma neurotoxicity assays are based on primary cultures of nerve cells for greater predictivity. Neurotoxic effects are quantified using either automated high-content imaging or live-content imaging, for accurate and fast toxicity screens.

CUSTOM RODENT PRIMARY NEURONAL CULTURES

Porsolt has a specific expertise in the development of rodent primary nerve cell cultures.

The following primary cultures have already been performed:

> Anterior pituitary gland     > Cortex

> Hippocampus                  > Hypothalamus

> Mesencephalon               > Striatum

> Vomeronasal organ         > Motoneurons

Neurite outgrowth assay: After drug treatment, neurons are immunostained with anti-MAP2 antibody to reveal the neurites  extension . Representative parameters of neuritic growth are generated and analyzed→

EARLY MARKERS OF NEUROTOXICITY OR TRADITIONAL CYTOTOXICITY ASSAYS

> Cell-based assay to monitor early markers of neurotoxicity: Neurite outgrowth assays | Calcium flux assays

> Standard cytotoxicity assays: Cytolysis | Mitochondrial membrane potential 

Quantification of glutamate toxic effects on mitochondrial membrane potential in primary cortical neuron cultures. Glutamate induces considerable mitochondrial depolarization through NMDA receptors, which is completely prevented by MK801 →