TARGETING TUMOR CELLS

ONCOLOGY

Oncology is an area that commands a larger proportion of the research world's resources. Porsolt is now able to provide services using its Oncology in vitro capabilities and expertise through the acquisition of Fluofarma.

TARGETING TUMOR CELLS

3D SHEROID ASSAYS

➠ For screening and efficacy studies — kinetic analysis

Analyze the efficacy of your compounds on tumor viability in a 3D relevant in vitro assay

The spheroid tumor models are composed of cells in different proliferative and metabolic states.

The spheroid culture mimics cellular interactions and morphology existing in tumors in vivo.

This 3D model ensures better predictivity when compared to 2D models for testing of new anticancer strategies.

CAM MODEL

The Chick Chorio Allantoic Membrane (CAM) model has raised interest over recent years as a substitute for murine cancer models as part of a 3R approach. The CAM model provides ideal microenvironment with high angiogenesis and matrix remodeling.

The non-fully functional lymphoid immune system of the embryo allows for the efficient xenograft of multiple types of tumor (prostate, glioma, colon, breast, liver…) and associated cell lines.

The model can be successfully applied to study cancer progression, pharmacological treatment or angiogenesis. The rich vascular network of the CAM model makes it an ideal system for angiogenesis studies related to cancer or other indications

HOLLOW FIBER MODEL

➠ A novel alternative for Murine classical xenograft models

The Hollow fiber model is an interesting substitute for murine classical xenograft models as part of a 3R approach.

The Hollow fiber model allows for the testing of several combinations of cell types in independent fibers in two different mouse compartments (subcutaneous or intraperitonal) using reduced number of mice.

Importantly, fibers can be used in fully immunocompetent mice which are less expensive than immunodeficient mice required for xenograft.

OUR PORTFOLIO

IN VITRO

NEW !

Cell viability colorimetric assay (2D)

 Multiple cellular models

ONC 10

NEW!

Cell viability - live-content imaging

Hanging drop and ultra low adherence

Multiple 2D cellular models

Multiple 3D cellular

PF 10.47
Clonogenicity HeLa/HCT-116 cells  In development

Cell cycle - cytometry endpoint analysis

Hanging drop and ultra low adherence

 Multiple 2D cellular models 

Multiple 3D cellular models

 PF 3.8

NEW !

Cell proliferation live-content imaging and cytometry end-point analysis

Hanging drop and ultra low adherence

Multiple 2D cellular models

Multiple 3D cellular models

PF 3.9

Migration - live-content imaging

Multiple cellular models PF 3.14
Invasion (3D) Multiple cellular models PF 3.15
IN OVO
Lung experimental metastasis model Multiple cellular models in chicken eggs ONC 4
IN VIVO
Hollow fiber assay Multiple cellular models in mouse ONC 5
Syngeneic model of breast cancer 4T1 cells in BALB/c mouse ONC 2
Syngeneic model of colon cancer CT26.WT cells in BALB/c mouse ONC 3
Syngeneic model of glioma (brain tumor) Mouse In development
Xenograft model of breast cancer MDA-MB -231cells in  BALB/c-nu mouse In development
Xenograft model of colon cancer HCT-8 cells in BALB/c-nu mouse In development
Xenograft model of glioblastoma (brain tumor) U118MG cells in BALB/c-nu mouse In development
Xenograft model of melanoma
(skin tumor)
A375 cells in BALB/c-nu mouse In development
Intravital imaging solution for CAM assay MDA-MB-231-RFP-Luc in chicken eggs In development
EX VIVO
Immunohistochemistry Multiples cells or tissue sections Contact us