PRIMARY TUMOR

➠ For screening and efficacy studies — kinetic analysis

Analyze the efficacy of your compounds on tumor viability in a 3D relevant in vitro assay

The spheroid tumor models are composed of cells in different proliferative and metabolic states.

The spheroid culture mimics cellular interactions and morphology existing in tumors in vivo.

This 3D model ensures better predictivity when compared to 2D models for testing of new anticancer strategies.

The Chick Chorio Allantoic Membrane (CAM) model has raised interest over recent years as a substitute for murine cancer models as part of a 3R approach. The CAM model provides ideal microenvironment with high angiogenesis and matrix remodeling.

The non-fully functional lymphoid immune system of the embryo allows for the efficient xenograft of multiple types of tumor (prostate, glioma, colon, breast, liver…) and associated cell lines.

The model can be successfully applied to study cancer progression, pharmacological treatment or angiogenesis. The rich vascular network of the CAM model makes it an ideal system for angiogenesis studies related to cancer or other indications

➠ A novel alternative for Murine classical xenograft models

The Hollow fiber model is an interesting substitute for murine classical xenograft models as part of a 3R approach.

The Hollow fiber model allows for the testing of several combinations of cell types in independent fibers in two different mouse compartments (subcutaneous or intraperitonal) using reduced number of mice.

Importantly, fibers can be used in fully immunocompetent mice which are less expensive than immunodeficient mice required for xenograft.