ONCOLOGY
Oncology is an area that commands a larger proportion of the research world's resources. Porsolt is now able to provide services using its Oncology in vitro capabilities and expertise.
➠ For screening and efficacy studies — kinetic analysis
Analyze the efficacy of your compounds on tumor viability in a 3D relevant in vitro assay
The spheroid tumor models are composed of cells in different proliferative and metabolic states.
The spheroid culture mimics cellular interactions and morphology existing in tumors in vivo.
This 3D model ensures better predictivity when compared to 2D models for testing of new anticancer strategies.
The Chick Chorio Allantoic Membrane (CAM) model has raised interest over recent years as a substitute for murine cancer models as part of a 3R approach. The CAM model provides ideal microenvironment with high angiogenesis and matrix remodeling.
The non-fully functional lymphoid immune system of the embryo allows for the efficient xenograft of multiple types of tumor (prostate, glioma, colon, breast, liver…) and associated cell lines.
The model can be successfully applied to study cancer progression, pharmacological treatment or angiogenesis. The rich vascular network of the CAM model makes it an ideal system for angiogenesis studies related to cancer or other indications
➠ A novel alternative for Murine classical xenograft models
The Hollow fiber model is an interesting substitute for murine classical xenograft models as part of a 3R approach.
The Hollow fiber model allows for the testing of several combinations of cell types in independent fibers in two different mouse compartments (subcutaneous or intraperitonal) using reduced number of mice.
Importantly, fibers can be used in fully immunocompetent mice which are less expensive than immunodeficient mice required for xenograft.
IN VITRO | ||
Cell viability colorimetric assay |
Multiple 2D cellular models |
ONC 10.1 |
Cell proliferation/cytolysis assay - high-content imaging |
Multiple 2D cellular models |
ONC 10.2 |
NEW ! Spheroid proliferation/cytolysis assay - high-content imaging |
Multiple 3D cellular models |
ONC 10.3 |
NEW ! Clonogenicity assay (anchorage-independent) |
Multiple 3D cellular models |
ONC 10.4 |
NEW ! Sphere formation and viability assay - high-content imaging |
Multiple 3D cellular models (Cancer stem cells) |
ONC 10.5 |
Cell cycle - cytometry analysis |
Multiple 2D or 3D cellular models |
PF 3.8 |
Migration assay - high-content imaging |
Multiple 2D cellular models |
PF 3.14 |
Invasion assay |
Multiple 3D cellular models |
PF 3.15 |
IN OVO | ||
Chicken Chorioallantoic Membrane (CAM) xenograft assay Multiple (screening – 3R approach) |
Multiple cellular models in chicken eggs | ONC 4 |
IN VIVO | ||
Hollow fiber assay (screening – 3R approach) | Multiple cellular models in mouse | ONC 5 |
Subcutaneous syngeneic model of: | ||
Breast cancer | 4T1 cells - Mouse | ONC 2.1 |
Colon cancer | CT26.WT/C26 cells - Mouse | ONC 2.2 |
Glioblastoma (brain tumor) | GL261 cells - Mouse | ONC 2.3 |
NEW! Orthotopic syngeneic models of: |
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Breast cancer |
4T1 cells - Mouse | ONC 3.1 |
Colon cancer |
CT26.WT/C26 cells - Mouse | ONC 3.2 |
Glioblastoma (brain tumor) |
GL261 cells- Mouse | ONC 3.3 |
Kidney cancer |
RenCa cells - Mouse | ONC 3.4 |
Lung cancer |
LLC1/KNL205 cells - Mouse | In development |
Liver cancer |
Hepa1-6/Hep-55.1C cells - Mouse | In development |
Stomach cancer |
CLS103 cells - Mouse | In development |
Ovarian cancer |
TERA-C3H cells - Mouse | In development |
Melanoma |
B16F10/S91/YUMM1.7 cells Mouse |
In development |
Osteosarcoma |
K7M2 wt cells Mouse |
In development |
Subcutaneous xenograft models of: | ||
Breast cancer | MDA-MB-231/ BT-20 cells - Mouse | ONC 6.1 |
Colon cancer | HCT-8/HCT-116/LoVo cells - Mouse | ONC 6.2 |
Glioblastoma (brain tumor) | U118MG/U87MG/M059J - Mouse | ONC 6.3 |
Kidney cancer | ACHN cells - Mouse | ONC 6.4 |
Lung cancer | A549/PC-9 cells - Mouse | ONC 6.5 |
Liver cancer | Hep3B2.1-7/HepG2 cells - Mouse | ONC 6.6 |
Ovarian cancer | OVCAR-3 cells - Mouse | ONC 6.7 |
Melanoma (skin tumor) | A375/MeWo cells - Mouse | ONC 6.8 |
Fibrosarcoma | HT-1080 cells - Mouse | ONC 6.9 |
Prostate cancer | LNCaP/PC-3/DU145 cells - Mouse | ONC 6.10 |
Pancreatic cancer | BxPC-3/PANC-1 cells - Mouse | ONC 6.11 |
Neuroblastoma | SH-SY5Y cells - Mouse | ONC 6.12 |
Bladder cancer | SW780 cells - Mouse | ONC 6.13 |
NEW! Orthotopic xenograft models of: | ||
Breast cancer | MDA-MB -231 cells - Mouse | ONC 7.1 |
Colon cancer | HCT-116/HCT-8 cells - Mouse | In development |
Lung cancer | A549/PC-9 cells - Mouse | In development |
Liver cancer | HepG2 cells - Mouse | In development |
EX VIVO | ||
Immunohistochemistry on tissue sections | Multiples biomarkers - Multiplex analysis | Contact us |