ONCOLOGY

ONCOLOGY

Oncology is an area that commands a larger proportion of the research world's resources. Porsolt is now able to provide services using its Oncology in vitro capabilities and expertise.

IN VIVO MODELS

Lung experimental metastasis model > Learn more Mouse ONC 1  NEW !
Chick Chorioallantoic Membrane (CAM) Assay Chicken eggs In development
Hollow fiber assay Mouse In development
Syngeneic model of breast cancer > Learn more 4T1 cells in balb/c mouse ONC 2  NEW !
Syngeneic model of colon cancer > Learn more CT26.WT cells in balb/c mouse ONC 3  NEW !

IN VITRO MODELS

We provide high-content analysis to support drug discovery projects in oncology, either in cellular models of human tumors in 2D or 3D cultures, or directly in tumor tissues and blood samples. We assist pharmaceutical companies and academic researchers in the discovery of new cancer drug targets by combining a panel of ready-to-use validated assays and over 10-years experience in assay development, to identify new anticancer compounds, and the quantitative analysis of novel cancer biomarkers.

CANCER READOUTS

NUCLEAR SIGNALING, DNA DAMAGE & CELL PROLIFERATION ASSAYS
p53 assay Topoisomerase I assay
Clonogenic assay Topoisomerase II assay
γH2AX assay Histone H3 acetylation assay
p21 assay Cell proliferation assay
Mdm2 assay
INFLAMMATION, ANGIOGENESIS & METASTASIS ASSAYS
Cytokine and chemokine assays NF-kB/p65 assay
STAT 1, 2, 3, 6 assay Mitochondrial depolarization test
iNOS activity assay Reactive oxygen species (ROS) assay
COX-2 activity assay Cell proliferation assays
LDL uptake assay Cell migration assays
Nur77 assay E-cadherin assay
B-Cell Linker (BLNK) assay In vitro angiogenesis assay

APOPTOSIS, PYROPTOSIS, NECROSIS ASSAYS
Caspase-1 assay Bax assay
Caspase 1,5 activation assay PARP1 enzyme activity assay
Caspase-3 assay NF-kB/p65 assay
Caspase-7 assay XIAP assay
Caspase-9 assay Reactive oxygen species (ROS) detection
Mitochondrial depolarization assay Calpain activity assay
Cytolysis assay TUNEL assay
Cytochrome c release assay Cytotoxicity assay

CANCER SIGNALING PATHWAYS & PHENOTYPIC ASSAYS
ERK assay c-Jun test
Ca2+ assay HIF-1a assay
cAMP assay NF-kappaB/p65
Phospho-CREB assay STAT1, 2, 3, 6 assay
mTOR assay EGFR assay
Akt assay Cell cycle assays
p38 activity assay Clonogenicity assay
JNK assay Cell migration assays

ENERGY METABOLISM & AUTOPHAGY ASSAY
NAD(P)H  / FAD assay Lysosome detection assay
ATP content assay mTOR activity assay

Mitochondrial membrane

potential assay

 Poly-ubiquitination assay
Reactive oxygen species test Proteasome activity assay
cAMP assay LC3B autophagy assay