Obesity

OBESITY & METABOLIC DISORDERS

Obesity and metabolism related disorders are key therapeutic areas that have attracted global attention over recent years. Porsolt has in place a comprehensive range of models for obesity, impaired glucose tolerance, and diabetes to assist with the development of novel therapeutic agents for the treatment and reduction of risk factors associated with metabolic diseases.

Obesity

Porsolt offers several rodent models for screening and testing the therapeutic potential of novel drugs to treat obesity.

Animal models:

ob/ob mice, db/db mice, Zucker fatty fa/fa rats, Zucker Diabetic Fatty (ZDF) rats, Diet-induced obese (DIO) mice,...

Assessments:

Body weight, Food/caloric intake, Glucose/Insulin levels, Glucose/Insulin tolerance tests, Metabolic biomarkers,…

Portfolio

Genetic animal models
ob/ob mice 

Leptin-deficient mouse: obesity, type 2 diabetes

db/db mice

Leptin-deficient mouse: obesity, type 2 diabetes 

Zucker Fatty rats

 Obesity, hyperlipidemia… 
Zucker Diabetic Fatty (ZDF) rats Type 2 diabetes, glucose intolerance, hyperinsulinemia...
Nutritional animal model

Diet-induced obesity (DIO)

 Obesity through conditioning with a special diet

Assessments
Fast-induced feeding (over 4 hours) Mouse MET 13
Acute 24-hr feeding Rat MET 14
Food/water intake and body weight gain (3-hr schedule-fed over 10 days) Rat MET 6
Food/water intake and body weight gain (over 28 days in pathotologic animals) Mouse - Rat MET 7

and  Oral or Intravenousglucose tolerance test  (in normal or pathologic animals) | Insulin tolerance test

Biochemistry

HOMA-IR, QUICKI and ISI calculation

Lipid panel (Triglycerides, Cholesterol, Free Fatty Acids,…)

Liver function panel (AminoTransferases, Alkaline Phosphatase, Bilirubin)

Renal function panel (Creatinine, Electrolytes,…)

Metabolic biomarkers (Adiponectin, Leptin, Glucagon,…)

Organ & Tissue collection

Acute feeding models (mouse or rat)

These studies are ideal for screening the effects of a test substance on food consumption in mice or rats and have a quick turn around time. Additional parameters to be measured can include glucose, insulin, lipid profile and body fat mass (*).
(*) DEXA Scan in collaboration with Atlantic Bone Screen (Nantes, France)

Effects of sibutramine on food intake in C57BL6 mice (after overnight fasting)

Effects of rimonabant and sibutramine on food intake in SD rats (reverse phase light-dark cycle)

Chronic feeding models

Models of chronic feeding measuring body weight and food intake.

• 3-hr schedule feeding for 10 days in normal rats:

Effects of sibutramine on body weight and food intake in Wistar rats

• Continuous feeding for 28 days in Zucker fa/fa rats:

Effects of sibutramine on body weight and food intake in Zucker fa/ fa rats