OBESITY & METABOLIC DISORDERS
Obesity and metabolism related disorders are key therapeutic areas that have attracted global attention over recent years. Porsolt has in place a comprehensive range of models for obesity, impaired glucose tolerance, and diabetes to assist with the development of novel therapeutic agents for the treatment and reduction of risk factors associated with metabolic diseases.
Porsolt offers several rodent models for screening and testing the therapeutic potential of novel drugs for the treatment and the reduction of risk factors associated with metabolic diseases.
Animal models:
ob/ob mice, db/db mice, Zucker fatty fa/fa rats, Zucker Diabetic Fatty (ZDF) rats, Diet-induced obese (DIO) mice, Streptozotocin (STZ)-induced diabetes, Alloxan-induced diabetes …
Assessments:
Body weight, Food/caloric intake, Glucose/Insulin levels, Glucose/Insulin tolerance tests, Metabolic biomarkers,…
Genetic animal models |
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ob/ob mice |
Leptin-deficient mouse: obesity, type 2 diabetes |
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db/db mice |
Leptin-deficient mouse: obesity, type 2 diabetes |
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Zucker Fatty rats |
Obesity, hyperlipidemia… | |
Zucker Diabetic Fatty (ZDF) rats | Type 2 diabetes, glucose intolerance, hyperinsulinemia... | |
Assessments |
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Oral glucose tolerance test (OGTT) (in normal or pathologic animals) | Mouse - Rat | MET 12 |
Intravenous glucose tolerance test (IVGTT) | Rat | MET 1 |
Insulin tolerance test (ITT) | Mouse - Rat | MET 2 |
and Body weight gain | Food, water or caloric intake |
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Biochemistry |
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HOMA-IR, QUICKI and ISI calculation Lipid panel (Triglycerides, Cholesterol, Free Fatty Acids,…) Liver function panel (AminoTransferases, Alkaline Phosphatase, Bilirubin) Renal function panel (Creatinine, Electrolytes,…) Metabolic biomarkers (Adiponectin, Leptin, Glucagon,…) |
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Organ & Tissue collection |
Measurement of fasting blood glucose levels before and after glucose challenge in Wistar or Sprague Dawley rats, or C57BL6 mice after overnight fasting. Results can be available within two weeks of receipt of the test substance.
OGTT in rats: effects of metformin
IVGTT in rats: effects of clozapine
OGTT in mice: effects of sitagliptin
Measurements of fasting blood glucose and plasma insulin levels before and after glucose challenge in ZDF rats.
OGTT in ZDF rats : effects of metformin and rosiglitazone
> Additional models: db-db mice, ob-ob mice, DIO mice and Zücker fa/fa rats.
> References: Metformin, Glibenclamide, Sitagliptin