OBESITY & METABOLIC DISORDERS
Obesity and metabolism related disorders are key therapeutic areas that have attracted global attention over recent years. Porsolt has in place a comprehensive range of models for obesity, impaired glucose tolerance, and diabetes to assist with the development of novel therapeutic agents for the treatment and reduction of risk factors associated with metabolic diseases.
Porsolt offers in vitro models and several in vivo models for screening and testing the therapeutic potential of novel drugs to treat diabetes.
Animal models:
ob/ob mice, db/db mice, Zucker Diabetic Fatty (ZDF) rats, Streptozotocin (STZ)-induced diabetes, Alloxan-induced diabetes …
Assessments:
Body weight, Food/caloric intake, Glucose/Insulin levels, Glucose/Insulin tolerance tests, Metabolic biomarkers,…
in vitro model |
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NEW! Glucose stimulated insulin secretion |
Rat insulinoma INS-1 cells | PF 7.2 |
Chemically-induced animal models | ||
Streptozotocin (STZ)-induced diabetes (rats or mice) | Type 1 diabetes, single injection of streptozotocin | |
Alloxan-induced diabetes (rats) | Type 1 diabetes, single injection of alloxan | |
HFD/STZ-induced diabetes (rats) | Type 2 diabetes | |
Genetic animal models |
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ob/ob mice |
Leptin-deficient mouse: obesity, Type 2 diabetes |
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db/db mice |
Leptin-deficient mouse: obesity, Type 2 diabetes |
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Zucker Diabetic Fatty (ZDF) rats | Type 2 diabetes, glucose intolerance, hyperinsulinemia... | |
Assessments |
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Oral glucose tolerance test (OGTT) HOMA-IR, QUICKI and ISI calculation |
Mouse – Rat | MET 12 |
Intravenous glucose tolerance test (IVGTT) | Rat | MET 1 |
Insulin tolerance test (ITT) | Mouse –Rat | MET 2 |
and Body weight gain | Food, water or caloric intake |
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Biochemistry |
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HOMA-IR, QUICKI and ISI calculation Lipid panel (Triglycerides, Cholesterol, Free Fatty Acids,…) Liver function panel (AminoTransferases, Alkaline Phosphatase, Bilirubin) Renal function panel (Creatinine, Electrolytes,…) Metabolic biomarkers (Adiponectin, Leptin, Glucagon,…) |
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Organ & Tissue collection |
Sprague Dawley rats fed a standard diet (SD) and given a single administration of streptozotocin (STZ 45 mg/kg). Effects of subchronic treatment (from Day 24 to Day 28 post STZ) in selected diabetic rats.
Basal glycemia
OGTT at Day 28
> Additional parameters: plasma insulin, urinary volume, glomerular filtration rate, food/water intake
> References: Metformin, Insulin
The diabetic profile of ZDF rats, 10-16 weeks of age, is characterized by increased body weight, hyperglycemia, hyperinsulinemia, glucose intolerance increasing in time, hyperphagia, polydipsia, polyuria, decrease in glomerular filtration rate (GFR), high levels of cholesterol and triglycerides.
Basal blood glucose and plasma insulin in ZDF rats at 10 and 16 weeks old
OGTT in ZDF rats aged 10 and 16 weeks old: effects of sitagliptin