OBESITY & METABOLIC DISORDERS

OBESITY & METABOLIC DISORDERS

Obesity and metabolism related disorders are key therapeutic areas that have attracted global attention over recent years. Porsolt has in place a comprehensive range of models for obesity, impaired glucose tolerance, and diabetes to assist with the development of novel therapeutic agents for the treatment and reduction of risk factors associated with metabolic diseases.

CELL-BASED ASSAYS RELATED TO METABOLIC DISORDERS & DIABETES

Quantification of lipid droplets in differentiated 3T3-L1 adipocytes

in vitro models based on primary cell cultures or cell lines:

Adipocytes, hepatocytes, pancreatic cells, etc...

New model of Glucose stimulated insulin secretion in Rat insulinoma INS-1 cells (internal reference PF 7.2)

>  Metabolism assays

lipolysis assays, lipids and phospholipids accumulation, lipid peroxidation, LDL uptake, NADH/FAD content, ATP content, intracellular ROS, P-glycoprotein (P-gp) activity, intracellular Glutathione (GSH) content, hepatobiliary function, ...

>  Neuroendocrinology-related assays

prolactin release and dopamine metabolism, glucocorticoid receptor translocation, etc...

IN VIVO SERVICES FOR METABOLIC DISORDERS

We offer to perform in vivo studies in predictive models of metabolic syndrome:

> Genetic and diet-induced in vivo models of obesity (Ob/Ob genetic model and high fat diet-induced obesity model) and diabetes (Db/Db genetic model and high fat diet-induced diabetes model)

Oral glucose tolerance test (OGTT) (in normal or pathologic animals) Mouse - Rat MET 12
Intravenous glucose tolerance test (IVGTT) Rat MET 1
Insulin tolerance test (ITT) Mouse - Rat MET 2
Metabolic Disorders - Learn More

> LC-MS/MS analysisDetection of metabolic factors, cytokines, etc., on tissue lysate or blood & derivatives (PBMC & blood samples, monocytes, macrophages,...)

> High content histology: Automated tissue imaging and multiplexed analysis of key markers with single-cell resolution (biomarker expression levels & subcellular localization).

OBESITY

Genetic Animal models
Leptin-deficience ob/ob - db/db  => obesity, type 2 diabetes Mouse MET 7
Zucker Fatty  => obesity, hyperlipidemia… Rat MET 7

Nutritional Animal models

Diet-induced obesity (DIO)

special diets

Mouse MET 18
Assessments
Fast-induced feeding (over 4 hours) Mouse MET 13
Acute 24-hr feeding Rat MET 14

Food/water intake and body weight gain (3-hr schedule-fed over 10 days)

Rat MET 6
Food/water intake and body weight gain (over 28 days in pathotologic animals) Mouse - Rat MET 7
Obesity - Learn More

DIABETES

In vitro model
NEW!   Glucose stimulated insulin secretion  Rat insulinoma INS-1 cells PF 7.2
Chemically-induced animal models
Streptozotocin (STZ)-induced type1 diabetes - single injection of streptozotocin Mouse – Rat MET 16
Alloxan-induced type 1diabetes - single injection of alloxan Rat MET 17
HFD/STZ-induced Type 2 diabetes - high fat diet and single injection of streptozotocin Rat MET 15
Genetic Animal Models
Zucker Diabetic Fatty (ZDF)
=> type 2 diabetes, glucose intolerance, hyperinsulinemia…
Rat MET 12
Nutritional Animal Models

Diet-induced obesity (DIO)

special diets

Mouse MET 18
Assessments
Oral glucose tolerance test (OGTT)
HOMA-IR, QUICKI and ISI calculation
Mouse – Rat MET 12
Intravenous glucose tolerance test (IVGTT) Rat MET 1
Insulin tolerance test (ITT)  Mouse –Rat MET 2
Diabetes - Learn More