LIVER & HEPATIC SYSTEM

LIVER & HEPATIC SYSTEM

Porsolt's experience in preclinical safety and efficacy has led to the development of models that focus on the liver and hepatic system. This is an important part of the development process to better understand the effects of compounds on the liver and hepatic system.

IN VITRO

Starting with a core expertise in polarized primary cultures of rat hepatocytes, Fluofarma , our affiliate, set up a tool box to support to support drug discovery in hepatology.

From cell-based screening up to biomarker studies in liver tissues, we offer quantitative and automated approaches to characterize the effects of drug candidates on liver functions and explore their mechanism of action (MOA).

LDL uptake

 HepG2 cell line

PF 3.10

Cholestasis / Bile canaliculi network

 Primary hepatocytes - sandwich configuration

PF 3.16

Glutathione (GSH), intracellular GSH content

 Multiple cellular models

PF 3.28

Acetaminophen toxicity model (GSH contents, cytolysis)

 Mouse primary hepatocytes

PF 3.41

Steatosis/Lipid, intracellular accumulation: neutral lipids

 Multiple cellular models

PF 3.29

NASH model Intracellular lipid accumulation

Primary Human and primary mouse hepatocytes

PF 6.1

↓ NASH Model - Test Sheet

Lipid, intracellular accumulation: phospholipids

 Multiple cellular models

PF 3.30

Mitochondrial Membrane Potential

 Multiple cellular models

PF 3.3

MRP2 expression

 Primary hepatocytes - sandwich configuration - hepatic tissue

PF 3.5

Cytolysis

 Multiple cellular models

PF 3.4

NQO1 expression

 Multiple cellular models

PF 3.24

Heme Oxygenase 1 expression

 Multiple cellular models

PF 3.25

NAD(P)H / FAD

 Multiple cellular models

PF 3.31

Hepatology - Learn more

IN VIVO

Carbon tetrachloride (CCl4) (acute model)

 Rat

LI 1

Acetaminophen (acute model)

 Mouse

LI 2

Bile Duct Ligation (BDL) (chronic model)

 Rat CV 2.7

NEW !

Non-alcoholic steatohepatitis (NASH) model

Mouse - Rat

In development

Focus on Carbon tetrachloride (CCl4) acute model in rats

Carbon tetrachloride (CCl4) is an hepatic toxin widely used for experimental induction of liver injury and oxidative stress.