in vitro dermatology

DERMATOLOGY

Dermatological diseases represent a major health problem due to the substantial patient population that is affected. Psoriasis, Allergic Contact Dermatitis (ACD) and Atopical Dermatitis (Allergic or Irritant Contact Dermatitis) are the major skin diseases of immunological origin.

in vitro dermatology

in vitro cell migration

Cell migration is central to key biological functions such as embryogenesis, immune response and inflammation. Porsolt developed standardized, sensitive and robust cell migration assays to study its underlying mechanisms and test novel therapeutic strategies.

> No reagents: cell migration is triggered by removing a strip of cells at the center of the well, using a 96-well wound maker tool.

> Kinetic monitoring: cell migration can be monitored over several days by label-free live-content imaging.

> Quantitative: cell migration is quantified by measuring cell density within the wound.

> Cell invasion can be addressed using similar approach based on matrigel matrix

in vitro angiogenesis

Our assay is based on the ability of endothelial cells to form capillary-like tubes in a co-culture model of fibroblasts and GFP-expressing HUVECs:

> Cells cultured in a specifically designed medium
> Angiogenesis is monitored by live content time-lapse imaging for the identification and visualization of HUVEC tube formation
> Tube formation is analyzed using an appropriate angiogenesis module, for robust determination of the total tube length
> Our angiogenesis assay enables to characterize compounds' effects in both dose- and time-dependent manner.