NEUROBIOLOGY

CENTRAL NERVOUS SYSTEM

Porsolt offers models in all areas of psychopharmacology, epilepsy, sleep-wake, and neurodegenerative disorders. We are uniquely placed to offer a full range of CNS efficacy and safety pharmacology assessments, from basic models and regulatory tests, through the evaluation of abuse and dependence liability and proconvulsant risk using EEG.

NEUROBIOLOGY

Based on more than 10-year of experience in the generation of tissue-specific rodent primary cell cultures, Porsolt offers to perform high-content screening in primary cultures of neurons and glial cells from the brain region of your choice.

Highly predictive, primary nerve cell cultures are powerful tools to characterize neuroprotective and neurotoxic effects, and study the mechanism of action of neuroactive compounds.

PREDICTIVE IN VITRO CNS MODELS IN HIGH-THROUGHPUT FORMAT

Directly isolated from specific brain regions of embryonic or newborn rodents, primary neuronal cultures mimic in vivo conditions:

➤ Various types of neurons and glial cells in mixed culture
➤ Neuronal-glial networks formation
➤ Expression of tissue-specific receptors

To combine high biological relevance with fast turnaround times, primary neuronal cultures are plated and grown in microplate format.

APPROPRIATE HIGH-CONTENT ANALYSIS TOOLS

➤ Live-content imaging: for kinetic neurite outgrowth and cell death monitoring

➤ High-content imaging: for quantification of subcellular events in specific cell subpopulations.

➤ Fluorometry: for high-throughput screening in homogenous neuronal cell populations

BRAIN TISSUE ANALYSIS

To study biomarkers and their topographic distribution, Porsolt couples tissue microarray (TMA) technology with automated tissue imaging.

Series of tissue specimens are assembled in the same TMA and analyzed simultaneously, thus ensuring optimal and reproducible results.

Porsolt‘s in-house capacities include:

➤ Brain tissue sampling & processing

➤ Brain TMA construction

➤ Multiplexed immunostaining (immunofluorescence or immunohistochemistry detection)

➤ Automated  imaging of tissue sections or TMAs

➤ Quantitative biomarker analysis: multiplexed quantification of biomarker expression levels and subcellular localization using dedicated image segmentation and data analysis software applications.