CENTRAL NERVOUS SYSTEM
Porsolt offers models in all areas of psychopharmacology, epilepsy, sleep-wake, and neurodegenerative disorders. We are uniquely placed to offer a full range of CNS efficacy and safety pharmacology assessments, from basic models and regulatory tests, through the evaluation of abuse and dependence liability and proconvulsant risk using EEG.
Epilepsy
| ACUTE EPILEPSY MODELS |
Generalized seizures:
> Electrical stimulation
Maximal Electroshock Test: tonic convulsions are induced by an electrical stimulation of high intensity.
Other test: Electroconvulsive Theshold Test (electrical stimulation of intermediate intensity)
> Chemical substance
Pentylenetetrazol seizures test: Pentylenetetrazol administered subcutaneously, induces clonic and tonic convulsions in the mouse or in the rat.
Other substances can be used to induce convulsions (bicuculline, strychnine or picrotoxin).
| MAXIMAL ELECTROSHOCK | Carbamazepine | Mouse/Rat | p.o. | yes |
| Diazepam | Mouse | p.o., i.p. | yes | |
| Lamotrigine | Rat | p.o. | yes | |
| Valproate | Rat | i.p. | yes | |
| ELECTROCONVULSIVE SHOCK THRESHOLD | Diazepam | Mouse/Rat | p.o., i.p. | yes |
| Valproate | Rat | i.p. | yes | |
| PENTYLENETETRAZOL-INDUCED CONVULSIONS | Diazepam | Mouse/Rat | p.o., i.p. | yes |
| Valproate | Rat | i.p. | yes | |
| TEST | REFERENCE | SPECIES | ROUTE | EFFECT |
Partial seizures:
> Electrical stimulation
6-Hz Psychomotor Test: electrical stimulation induces seizures characterized by forelimb clonus and Straub tail in the mouse .
| CHRONIC EPILEPSY MODELS |
Generalized seizures:
> Genetic animal model
DBA/2 mice are susceptible to audiogenic seizures and exibit wild running followed by clonic and tonic convulsions when exposed to highintensity sound.
Partial seizures:
> Electrical stimulation
Electrical Amygdala Kindling test: partial seizures with secondary generalization are induced by repeated electrical stimulation in a limbic region (amygdala for example).
| AUDIOGENIC SEIZURE | Mouse | Carbamazepine | p.o. | yes |
| Mouse | Diazepam | p.o., i.p. | yes | |
| Mouse | Lamotrigine | p.o. | yes | |
| Rat | Valproate | i.p. | yes | |
| ELECTRICAL AMYGDALA KINDLING | Rat | Carbamazepine | p.o. | yes |
| Rat | Diazepam | p.o. | yes | |
| Mouse | Valproate | i.p. | yes | |
| TEST | SPECIES | REFERENCE | ROUTE | EFFECT |
> Parameters measured
- Seizure score based on the Racine’s scale.
- Afterdischarge duration evaluated by EEG recording.
Porsolt
Z.A. de Glatigné
53940 Le Genest-Saint-Isle, France
Telephone +33 2 43 69 36 07
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