BRAIN TISSUE ANALYSIS

CENTRAL NERVOUS SYSTEM

Porsolt offers models in all areas of psychopharmacology, epilepsy, sleep-wake, and neurodegenerative disorders. We are uniquely placed to offer a full range of CNS efficacy and safety pharmacology assessments, from basic models and regulatory tests, through the evaluation of abuse and dependence liability and proconvulsant risk using EEG.

BRAIN TISSUE ANALYSIS

HIGH-CONTENT HISTOLOGY OF BRAIN TISSUES

To study biomarkers and their topographic distribution, Porsolt couples tissue microarray (TMA) technology with automated tissue imaging. Series of tissue specimens are assembled in the same TMA and analyzed simultaneously, thus ensuring optimal and reproducible results. Porsolt‘s in-house capacities include:

> Brain tissue sampling & processing

> Brain TMA construction

> Multiplexed immunostaining (immunofluorescence or immunohistochemistry detection)

> Automated  imaging of tissue sections or TMAs

> Quantitative biomarker analysis: multiplexed quantification of biomarker expression levels and subcellular localization using dedicated image segmentation and data analysis software applications.

TISSUE MICROARRAY

- Simultaneous processing of large experimental series
- Reduction of variability between specimens
- Standardization and optimization of biomarker staining (IF/IHC)
- Automated acquisition of stained TMA
- Fast identification and true quantification of biomarkers of interest
- TMA construction from a large variety of tissue materials

OVERVIEW OF BRAIN TISSUE PROCESSING