CENTRAL NERVOUS SYSTEM

CENTRAL NERVOUS SYSTEM

Porsolt offers models in all areas of psychopharmacology, epilepsy, sleep-wake, and neurodegenerative disorders. We are uniquely placed to offer a full range of CNS efficacy and safety pharmacology assessments, from basic models and regulatory tests, through the evaluation of abuse and dependence liability and proconvulsant risk using EEG.

NEUROBIOLOGY

IN VITRO

Neurite outgrowth

Primary neurons & neuronal cell lines

PF 3.6

γ-secretase activity

Established HeLa cell clone

PF 3.13

Prolactin release

Rat primary pituitary cells

PF 9.9

Microtubule dynamics in cellulo

Primary neurons & neuronal cell lines

PF 9.2

Autophagy (LC3B)

Multiple cellular models

PF 3.17

Proteasome 20S subunit quantification

Multiple cellular models

PF 9.8

Lipid peroxidation

Multiple cellular models

PF 3.18

Nrf2 activation

Multiple cellular models

PF 3.19

Tau Phosphorylation

Rat primary cortical neurons

PF 9.3

Amyloid Beta1-42 induced neurotoxicity

Rat primary cortical neurons

PF 9.10

Bacterial particles phagocytosis

Rat primary cortical neurons

PF 9.11

NF kB activation

Multiple cellular models

PF 3.23

Glucocorticoid receptor subcellular translocation

AtT20/Primary pituitary cells

PF 3.26

ERK activation (pERK1/2)

Multiple cellular models

PF 3.27

cAMP quantification

Multiple cellular models

PF 3.34

Calcium signaling (ionotropic and metabotropic receptor pharmacology)

Ionotropic and metaotropic receptor pharmacology

PF 3.33

Poly-Ubiquitin detection

Multiple cellular models

PF 3.28

Heme Oxygenase 1 expression

Multiple cellular models

PF 3.25

FKBP52

Multiple cellular models

PF 9.6

CREB phosphorylation

Multiple cellular models

PF 3.35

NeuroToxicity: gliosis

Primary cultures

PF 9.7

Neuromuscular junction quantification

Co-culture of mouse myotubes and primary spinal cord neurons

PF 9.4

Caspase 3/7 activity

Multiple established cell clones

PF 3.1

Caspase 3/7 activation

Multiple cellular models

PF 3.2

Mitochondrial Membrane Potential

Multiple cellular models

PF 3.3

Cytolysis

Multiple cellular models

PF 3.4

Intracellular reactive oxygen species

Multiple cellular models

PF 3.11

Calpain activity

Multiple established cell clones

PF 3.12

NQO1 expression

Multiple cellular models

PF 3.24

Monoamine oxidase B activity

HepG2 cell line

PF 9.1

Myelin Basic Protein (MBP) quantification

Multiple cellular models

PF 9.5

Neurobiology - Learn More

CNS GENERAL SCREENING

IN VIVO
Primary observation (Irwin) Mouse - Rat CNS 1.1
Activity meter Mouse - Rat CNS 1.2
Rotarod Mouse - Rat CNS 1.5
Accelerating rotarod Mouse - Rat CNS 1.17
Beam walking Mouse - Rat CNS 1.12
Foot-fault Rat CNS 1.16
Removal of adhesive Rat CNS 1.14
Neurological score Rat CNS 1.15
Barbiturate interaction (sleep induction) Mouse - Rat CNS 1.8
Ethanol interaction (sleep induction) Mouse - Rat CNS 1.9
Tetrad test Mouse  CNS 1.13
Rectal temperature (option: implants) Mouse - Rat CNS 1.11
Odor discrimination Rat CNS 1.18
Grip strength Mouse - Rat CNS 1.19
CNS General screening - Learn More

PSYCHIATRIC DISEASES

DEPRESSION

IN VIVO
Reserpine Mouse - Rat CNS 2.1
Behavioral despair Mouse - Rat CNS 2.5
Open space swimming Mouse CNS 2.8
Differential reinforcement of low rate (DRL 30) Rat CNS 2.6
Chronic Mild Stress Mouse CNS 2.10

ANXIETY

IN VIVO
Four plates Mouse CNS 3.1
Staircase Mouse CNS 3.2
Elevated plus-maze Mouse - Rat - Gerbil CNS 3.3
Light-dark box Mouse CNS 3.4
Novelty-induced hypophagia Mouse - Rat CNS 3.5
Stress-induced hyperthermia (group-housed animals) Mouse CNS 3.6
Stress-induced hyperthermia (singly-housed animals) Option: implants Mouse CNS 3.17
Chronic corticosterone model Mouse Partnership
Marble burying Mouse CNS 3.7
Vogel conflict Rat CNS 3.8
Fear potentiated startle reflex Rat CNS 3.13

PSYCHOSIS

IN VIVO
Amphetamine hyperactivity Mouse - Rat CNS 4.1
Amphetamine stereotypy Mouse - Rat CNS 4.2
Apomorphine climbing Mouse CNS 4.12
Low dose apomorphine Mouse CNS 4.5
Mescaline scratching Mouse CNS 4.6
MK-801 hyperactivity Mouse - Rat CNS 4.13
PCP hyperactivity Mouse - Rat CNS 4.8
Catalepsy Mouse - Rat CNS 4.9
Prepulse inhibition (deficit induced by apomorphine) Rat CNS 4.11
Prepulse inhibition (deficit induced by MK-801) Rat CNS 4.14
Prepulse inhibition (deficit induced by PCP) Rat CNS 4.15
Social investigation (deficit induced by MK-801) Rat CNS 4.16

NEW!

Neonatal PCP and Social Isolation (two-hit) model

Rat

In development

Psychiatric diseases - Learn More

EPILEPSY

IN VIVO
Maximal electroshock Mouse - Rat CNS 5.1
Electroconvulsive threshold Mouse - Rat - Gerbil CNS 5.2
Pentylenetetrazole seizures Mouse - Rat CNS 5.3
Intravenous PTZ seizure threshold Rat CNS 5.11
Strychnine convulsions Mouse - Rat CNS 5.4
Picrotoxin convulsions Mouse - Rat CNS 5.5
Bicuculline convulsions Mouse - Rat CNS 5.6
Kainic acid convulsions Rat CNS 5.10

NEW !

Lithium-pilocarpine-induced seizures

Rat

CNS 5.13

Audiogenic seizures Mouse CNS 5.7
6 Hz psychomotor Mouse - Rat - Gerbil CNS 5.9
GBL-induced absence epilepsy (EEG telemetry) Mouse CNS 5.12
Genetic absence epilepsy GAERS Rat Contact us
Mesial temporal lobe epilepsy MTLE Mouse Contact us
Electrical amygdala kindling (Electrophysiology) Rat CNS 9.3
Epilepsy - Learn More

MOTIVATION

IN VIVO

NEW !

Effort-Related Choice Behavior (operant)

Rat Partnership

NEW !

Effort-Related Choice Behavior (T Maze)

Rat Partnership

NEW !

Progressive Ratio Chow Feeding Choice Task (operant)

Rat Partnership

NEW !

Sociability (three - chamber) test

Mouse In development

COGNITION & DEMENTIA

IN VITRO

NEW !

Human drug and replicative induced senescence

Wi-38 cells

PF 9.12

IN VIVO EXPERIMENTAL PROCEDURES
Passive avoidance Mouse - Rat CNS 6.19
Morris water maze (single session) Rat CNS 6.16
Morris water maze (4 sessions + probe) Mouse - Rat CNS 6.17
Social recognition (30 minute retention) Rat CNS 6.20
Social recognition (120 minute retention) (delay-induced forgetting) Rat CNS 6.21
Delayed alternation (acquisition) Rat CNS 6.13
Delayed alternation (stabilized performance) Rat CNS 6.15
Operant reversal Rat CNS 6.24

NEW!

Fear Conditioning (context)

Mouse

In development

NEW!

Operant Set-shifting

Rat

In development

NEW!

Spontaneous Alternation

Mouse

In development

MODELS OF PHARMACOLOGICALLY- INDUCED AMNESIA

Scopolamine - induced amnesia
Passive avoidance Mouse - Rat CNS 6.1
Morris water maze (single session) Rat CNS 6.3
Morris water maze (4 sessions + probe) Rat CNS 6.18
Social recognition (30 minute retention) Rat CNS 6.5
Delayed alternation (stabilized performance) Rat CNS 6.28
Operant reversal Rat CNS 6.32
Diazepam - induced amnesia
Passive avoidance Mouse - Rat CNS 6.27
MK 801 - induced amnesia
Passive avoidance Rat CNS 6.26
Morris water maze (4 sessions + probe) Rat CNS 6.23
Social recognition (30 minute retention) Rat CNS 6.33
Delayed alternation (stabilized performance) Rat CNS 6.29
Operant reversal Rat CNS 6.31
PCP - induced amnesia
Delayed alternation (stabilized performance) Rat CNS 6.30
Operant reversal Rat CNS 6.37

AGED-RELATED DEFICIT

Social recognition Aged Rat CNS 6.9
Morris water maze (4 sessions + probe) Aged Rat CNS 6.7
Delayed alternation (acquisition) Aged Rat CNS 6.10
Delayed alternation (stabilized performance) Aged Rat CNS 6.11
Operant reversal Aged Rat CNS 6.34

NEW !

Spatial Memory (spontaneous alternation
and Morris water maze)

Aged Mouse

In development

Cognition & Dementia - Learn More

DRUG ABUSE & DEPENDENCE

IN VIVO
Naloxone-precipitated withdrawal (Saelens) Mouse CNS 7.1
Flumazenil-precipitated withdrawal (ECS threshold) Mouse CNS 7.2
Non-precipitated withdrawal (option: telemetry) Rat CNS 7.3
Opiate tolerance (hot plate) Mouse - Rat CNS 7.4
Place preference Rat CNS 7.5
Self-administration (initiation) Rat CNS 7.6
Self-administration (substitution) Rat CNS 7.7
Self-administration (reinstatment) Rat CNS 7.9
Self-administration (progressive ratio) Rat CNS 7.10
Drug discrimination Rat CNS 7.8
Drug abuse & Dependence - Learn More

ELECTROPHYSIOLOGY

CONCIOUS ANIMALS (TELEMETRY)
Quantified EEG Mouse - Rat CNS 9.7
Sleep/wakefulness cycle Rat CNS 9.2
Electrical amygdala kindling Rat CNS 9.3
EEG trace monitoring Mouse - Rat - Dog CNS 9.5
Electrophysiology - Learn More

NEURODEGENERATION & STROKE

IN VITRO
 Glutamate-induced exitotoxicity Rat cortical or hippocampal neurons CNS 10.13

HUNTINGTON DISEASE

IN VIVO
Motor function and neuroscore - Subchronic 3-NPA Rat CNS 10.8

  ↓ EXPERIMENTAL PROCEDURES

     Activity meter

     Rotarod
     Lesion volume

PARKINSON DISEASE

IN VITRO
6-OHDA-induced toxicity SH-SY5Y cells CNS 10.15

6-OHDA-induced toxicity
(Neurite outgrowth of dopamine neurons)

ICell® DopaNeurons CNS 10.16
MPP+ induced toxicity
(Neurite outgrowth and apoptosis)
iCell® DOPA neurons CNS 10.17
IN VIVO
Tremor
Tacrine-induced tremulous jaw-movements (TJM)
Rat CNS 10.1
Turning behavior
Unilateral medial forebrain bundle (MFB) 6-OHDA lesion
Rat CNS 10.7
Stepping behavior
Unilateral medial forebrain bundle (MFB) 6-OHDA lesion
Rat CNS 10.2
L-DOPA dyskinesia
Unilateral medial forebrain bundle (MFB) 6-OHDA lesion
Rat CNS 10.5
Cognitive deficit
Bilateral striatal 6-OHDA lesion
Rat CNS 10.9
Psychosis-like-behaviour
Bilateral striatal 6-OHDA lesion
Rat CNS 10.12
Parkinson's Disease - Learn More

ALZHEIMER DISEASE

IN VITRO
Tau hyperphosphorylation Rat cortical culture PF 9.3
Amyloid Beta1-42 induced neurotoxicity Rat cortical culture PF 9.10
IN VIVO
Streptozotocin (STZ) - induced cognitive deficit Rat CNS 10.11

     ↓ EXPERIMENTAL PROCEDURES

        Morris water maze

        Delayed alternation
   Alzheimer's Disease - Learn More

STROKE

IN VITRO
 NMDA-induced excitotoxicity  Rat cortical neurons CNS 10.21
IN VIVO
Intrastriatal NMDA administration   Mouse CNS 10.14

      ↓ EXPERIMENTAL PROCEDURES:

        Lesion volume

Transient focal cerebral ischemia
Middle Cerebral Artery Occlusion
Rat CNS 10.3

      ↓ EXPERIMENTAL PROCEDURES:

        Beam walking

        Foot-fault
        Removal of adhesive
        Neurological score
        Lesion volume
Stroke - Learn More