Cardiac electrophysiology

CARDIOVASCULAR SYSTEM

Porsolt has an extensive portfolio of cardiovascular procedures, ranging from standard cardiovascular telemetry studies for safety evaluation, to pathophysiological models for specific therapeutic areas. Porsolt also possesses considerable expertise with in vitro models, providing clients with a comprehensive assessment of all aspects of cardiovascular function.

Cardiac electrophysiology

While the hERG channel is still considered to be the main target for in vitro proarrhythmic risk assessment, a rapid and preliminary test of the L type calcium current (ICaL), the rapid component of the sodium current (INa) and the ionic currents responsible for maintaining resting membrane potential (the inward rectifier potassium current IK1) using the patch-clamp technique in transfected cells is recommended for drawing a more complete cardiac risk profile of a new drug.

hCav1.2 channel

Effects of nifedipine on the amplitude of ICa

hNav1.5 channel

Effects of tetracaine on the amplitude of INa

hKir2.1 channel

Effects of barium chloride on the amplitude of IK1

Left atria in the rabbit

Effect of lidocaine on conduction velocity in the rabbit and guinea-pig atria

Rabbit atria are more sensitive than rabbit Purkinje fibers or guinea-pig atria for detecting lidocaine-induced cardiac conduction slowing (Goineau et al., 2012).