VISIT US AT EUROTOX 2019

Porsolt will be present at the 54rd congress of the European Societies of Toxicology

Helsinki | Finland

September 8th-11th, 2019

Booth #26

Schedule a meeting

 

We will be presenting this poster :

Methotrexat-induced intestinal mucositis in the rat

P. Guillaume, F. Tantot, L. Lecouflet, V. Castagné, S. Goineau

Methotrexate (MTX) is used for the treatment of many diseases, including psoriasis, rheumatoid arthritis and various neoplastic diseases. Nevertheless, among various adverse effects MTX causes intestinal mucositis and diarrhea. Mucositis incidence can vary with the drug regimen, and we therefore aimed to investigate the effects of MTX in rats following a single or a repeated treatment.

Male Wistar rats (n=8/group) were injected with vehicle (PBS), MTX (7 mg/kg, i.p.) for 3 consecutive days or MTX (25 mg/kg, i.p.) once. Body weight and diarrhea (4-scale score) were assessed over the test period, and inflammatory and histopathological responses were investigated 5 days after the first injection. The potential protective effects of sucralfate were then evaluated in a separate experiment.

In contrast to controls, no gain in body weight was observed in both MTX regimens. A single treatment at 25 mg/kg did not induce signs of mucositis, while MTX (7 mg/kg/day for 3 days) increased the diarrhea score and its incidence rate compared to controls (+1.4 and 62.5%, respectively, p<0.05), increased the ileum MPO activity (+3640%, p<0.05), ileum TNF-α levels (+364%, p<0.001), tended to reduce ileum GSH levels (-25%, p=0.09) and induced a marked disruption of the intestinal architecture. The repeated administration of a 7 mg/kg MTX daily dose therefore induced a clear inflammatory response, a modification of mucosal integrity, and a moderate diarrhea, while a single administration of a higher MTX was inactive. Sucralfate (300 and 600 mg/kg, p.o. for 4 consecutive days), failed to reverse the effects of repeated MTX administrations.

These findings suggest that the repeated MTX treatment-induced gastrointestinal toxicity in rats shows similarities with clinical intestinal mucositis manifestations. This rat experimental mucositis model therefore offers a promising tool for evaluating the side effects of novel chemotherapeutic agents or the efficacy of potential treatments against chemotherapy-induced mucositis. The fact that sucralfate did not reverse MTX-induced mucositis is consistent with the absence of effective clinical treatment.

NEWS ARCHIVES

25/06/2019 Visit us at SPS 2019 READ THIS NEWS 25/06/2019 Meet us at ECNP congress 2019 READ THIS NEWS 25/06/2019 Meet us at EFIC 2019 READ THIS NEWS 24/06/2019 Visit us at SFN meeting 2019 READ THIS NEWS 24/06/2019 Visit us at Cosmetic 360 READ THIS NEWS 24/06/2019 Meet us at AFSTAL meeting 2019 READ THIS NEWS 13/06/2019 Meet us at CPDD / NIDA meeting READ THIS NEWS 23/05/2019 Visit us at Bio International 2019 READ THIS NEWS 20/05/2019 Meet us at the 4th Annual Advances in Immuno-Oncology Congress READ THIS NEWS 23/04/2019 Meet us at SPIN Congress 2019 ! READ THIS NEWS 23/04/2019 Meet us at EUCAS Congress 2019 ! READ THIS NEWS 23/04/2019 Meet us at Biotrinity Congress 2019 ! READ THIS NEWS 04/03/2019 Meet us at ADPD Congress 2019 READ THIS NEWS 04/03/2019 Meet us at Bio Europe Spring Congress 2019 READ THIS NEWS 15/01/2019 Meet us at 35th Scientific Day of AFERO 2019 READ THIS NEWS 19/12/2018 Porsolt team wishes you a Merry Christmas & a Happy New Year ! READ THIS NEWS 09/08/2018 Meet us at World Congress of Pain 2018 READ THIS NEWS 09/08/2018 Visit us at EuroTox 2018 READ THIS NEWS 09/08/2018 Visit us at BIOFIT 2018 READ THIS NEWS 09/08/2018 Visit us at Society of Neuroscience 2018 READ THIS NEWS 09/08/2018 Visit us at ACTox 2018 READ THIS NEWS 09/08/2018 Visit us at Bio Europe 2018 READ THIS NEWS 09/08/2018 Meet us at ECNP Congress 2018 READ THIS NEWS 09/08/2018 Visit us at NLS Days 2018 READ THIS NEWS 09/08/2018 Meet us at ITTC 2018 - CGO READ THIS NEWS 08/08/2018 Visit us at SAFETY PHARMACOLOGY 2018 READ THIS NEWS 26/03/2018 Meet us at NIDA Forum 2018 READ THIS NEWS 26/03/2018 Meet us at WPC 2018 READ THIS NEWS 26/03/2018 Visit us at Bio International 2018 READ THIS NEWS 26/03/2018 Visit us at BIOMED 2018 READ THIS NEWS 26/03/2018 Meet us at Immuno-Oncology Congress 2018 READ THIS NEWS 26/03/2018 Meet us at Advances in neurodevelopmental & neurodegenerative disorders READ THIS NEWS 06/02/2018 Visit us at SOT 2018 READ THIS NEWS 06/02/2018 Meet us at AD/PD conference 2018 READ THIS NEWS 26/01/2018 Porsolt Catalog 2018 ! READ THIS NEWS 21/12/2017 Happy Holidays from the Porsolt Team ! READ THIS NEWS 08/11/2017 Porsolt Newsletter - November 2017 READ THIS NEWS 01/11/2017 Visit us at BIOFIT 2017 READ THIS NEWS 01/11/2017 Meet us at Genesis 2017 READ THIS NEWS 18/09/2017 Visit us at Neuroscience READ THIS NEWS 18/09/2017 Visit us at ACTox READ THIS NEWS 18/09/2017 Visit us at Bio Europe READ THIS NEWS 30/05/2017 Visit us at Bio International 2017 READ THIS NEWS 30/05/2017 Meet us at On Helix 2017 READ THIS NEWS 30/05/2017 Visit us at ECNP Congress 2017 READ THIS NEWS 30/05/2017 Visit us at EuroTox 2017 READ THIS NEWS 30/05/2017 Meet us at EFIC 2017 READ THIS NEWS 30/05/2017 Visit us at SAFETY PHARMACOLOGY 2017 READ THIS NEWS 30/05/2017 Visit us at NLS Days 2017 READ THIS NEWS 24/02/2017 Meet us at Biotrinity 2017 READ THIS NEWS 24/02/2017 Meet us at Immuno-Oncology Congress READ THIS NEWS 24/02/2017 Visit us at BioMed 2017 READ THIS NEWS 24/02/2017 Meet us at NeuroFrance 2017 READ THIS NEWS 20/01/2017 Visit us at Bio-Europe Spring 2017 READ THIS NEWS 20/01/2017 Visit us at SOT 2017 READ THIS NEWS 20/01/2017 Visit us at AD/PD conference READ THIS NEWS 01/11/2016 Meet us at Genesis 2016 READ THIS NEWS 04/09/2016 Visit us at ACTox 2016 READ THIS NEWS 03/09/2016 Visit us at Neuroscience 2016 READ THIS NEWS 02/09/2016 Visit us at Bio-Europe 2016 READ THIS NEWS 01/09/2016 Meet us at ESTIV READ THIS NEWS 16/08/2016 Visit us at Safety Pharmacology 2016 joint meeting READ THIS NEWS 16/08/2016 Meet us at the Brain Conference 2016 READ THIS NEWS 16/08/2016 Meet us at ECNP Congress READ THIS NEWS 16/08/2016 Visit us at NLS Days READ THIS NEWS 16/08/2016 Visit us at the Innovation Days READ THIS NEWS 22/06/2016 Cardiac risk assessment in human induced pluripotent stem cell derived cardiomyocytes READ THIS NEWS 01/06/2016 Meet us at ESC 2016 READ THIS NEWS 29/03/2016 Meet us at FENS 2016 READ THIS NEWS 02/03/2016 Meet us at CPDD READ THIS NEWS 02/03/2016 Meet us the Alzheimer's Disease Congress READ THIS NEWS 01/03/2016 Meet us at EPHAR 2016 READ THIS NEWS 29/02/2016 Visit us at Bio 2016 READ THIS NEWS 29/02/2016 Visit us at IATI-Biomed 2016 READ THIS NEWS 26/02/2016 Meet us at ISDN 2016 READ THIS NEWS 11/02/2016 Visit us at Bio-Europe Spring 2016 READ THIS NEWS 18/12/2015 Season's Greetings READ THIS NEWS 10/12/2015 Porsolt expands its in vitro drug discovery service capabilities with the acquisition of Fluofarma READ THIS NEWS 02/11/2015 Visit us at SOT 2016 READ THIS NEWS 28/07/2015 Meet us at Prix Galien USA 2015 READ THIS NEWS 04/06/2015 Meet us at EFIC 2015 READ THIS NEWS 02/06/2015 Meet us at ESDR Annual Meeting READ THIS NEWS 02/06/2015 Visit us at Neurosciences READ THIS NEWS 02/06/2015 Visit us at Bio Latin America READ THIS NEWS 30/05/2015 Meet us at EAACI READ THIS NEWS 15/05/2015 Meet us at EAVPT READ THIS NEWS 03/01/2011 New laboratory READ THIS NEWS