Porsolt's high-content imaging platform is fully automated. It includes a robotic plate management system, an automated confocal microscope, and tailor-made software solutions for fast image segmentation and cytometric data analysis.
Particularly indicated for the performance of multiplexed phenotypic screening, automated high-content imaging affords a quantitative approach to cellular and subcellular morphological and molecular features, thus providing a deep understanding of the effects and mechanism of action of compounds.
> Spatial information: measurements of subcellular signals and relocalization in specific cell compartments
> Morphological parameters: quantification of cellular & subcellular organelle morphology
> Kinetics: short-term dynamic measurements in live cells
> Multiplexed analysis at single-cell level: automated image segmentation allows the multiplexed analysis of morphological features and signaling pathways.
High-content imaging enables the quantification of subcellular events in specific cell subpopulations. Here, ERK 1/2 phoshorylation (red) is detected in a specific neuronal population, within a primary striatal culture (green).
> Predictive cellular models: mono- or multi-cell type cellular models tailored to your need, based on cell lines, primary cell cultures or stem cells.
> 100 phenotypic & molecular assays for immediate performance or adaptation, complemented with assay development services
> In-house capacities in image segmentation & data analysis: customization of imaging softwares or development of new algorithms for image segmentation, and bespoke data analysis and visualization using our proprietary software solution: Cytosurfer®
INSTRUMENT | BD pathway 855 |
FORMAT | 96 and 384 well-plates |
BIOLOGICAL SAMPLES | fixed and live cells |
MULTIPLEXING | morphological parameters & IF signals |