HIGH CONTENT IMAGING

Porsolt's high-content imaging platform is fully automated. It includes a robotic plate management system, an automated confocal microscope, and tailor-made software solutions for fast image segmentation and cytometric data analysis. 

Particularly indicated for the performance of multiplexed phenotypic screening, automated high-content imaging affords a quantitative approach to cellular and subcellular morphological and molecular features, thus providing a deep understanding of the effects and mechanism of action of compounds.

KEY ADVANTAGES OF HIGH CONTENT IMAGING

> Spatial information: measurements of subcellular signals and relocalization in specific cell compartments

> Morphological parameters: quantification of cellular & subcellular organelle morphology

> Kinetics: short-term dynamic measurements in live cells

> Multiplexed analysis at single-cell level: automated image segmentation allows the multiplexed analysis of morphological features and signaling pathways.

High-content imaging enables the quantification of subcellular events in specific cell subpopulations. Here, ERK 1/2 phoshorylation (red) is detected in a specific neuronal population, within a primary striatal culture (green).

PORSOLT'S ADDED VALUE


> Predictive cellular models: mono- or multi-cell type cellular models tailored to your need, based on cell lines, primary cell cultures or stem cells.

> 100 phenotypic & molecular assays for immediate performance or adaptation, complemented with assay development services

> In-house capacities in image segmentation & data analysis: customization of imaging softwares or development of new algorithms for image segmentation, and bespoke data analysis and visualization using our proprietary software solution: Cytosurfer®

TECHNICAL FEATURES

INSTRUMENT BD pathway 855
FORMAT 96 and 384 well-plates
BIOLOGICAL SAMPLES fixed and live cells
MULTIPLEXING  morphological parameters & IF signals