To identify compounds modulating lipid metabolism (slimming claim) we perform, in differentiated adipocytes, a lipolysis activation assay multiplexing quantification of triglyceride content and glycerol release, and combining extracellular / intracellular measurements.

Evaluation of products or active ingredients that could act on adipose tissue for slimming activity :

- Lipolysis activation (adipocyte from 3T3-L1 cells differentiation)

Lipolysis activation in adipocytes

3T3-L1cells were differentiated into adipocytes. After complete differentiation, the compound is applied (24h-48h) and cells were stained for triglycerides content in parallel with glycerol release measurement in the cell culture medium. Isoproterenol (Iso) is used as a positive control for its known lipolytic activity.