Almost 80% of urban areas have levels of pollutants greater than the recommended levels of the World Health Organization (WHO). Given that 54% of the world population lives in urban areas, the number of people exposed to pollutants continues to increase, especially in big cities. Anti-pollution objectives are currently on top of the cosmetic industry agenda.

PORSOLT offers many in vitro models to evaluate:
- Cell viability measurement (NHDF, NHEK, HaCaT)
- Autophagy which is a key system to protect against pollution exposition (LC3B)
- Inflammatory cytokine release
- Reactive Oxygen species (ROS) and Lipid peroxidation measurement
- DNA damages

Protection against ROS generation and lipid peroxidation induced by pollutant

Numerous epidemiological studies have established a consistent association between urban dust particulate matter concentrations and increased skin troubles. Various experimental studies provide a correlation between urban dust oxidative capacity and its toxicity. Oxidative stress can damage lipids, proteins, membrane, DNA, and can cause cell death by necrotic or apoptotic processes.

We provide new assays to assess anti-pollution claim by challenging ingredients against ROS production (left graph) and lipid peroxidation (right graph) induced by urban dust in human keratinocytes.

Autophagy modulation

The importance of the autophagy process in a number of pathologies, in the aging process, and protection against pollution, is now an emerging trend.

Autophagy modulation is measured by immunostaining of endogenous LC3B, a widely used marker for autophagosome.

The LC3B turnover assay, performed in the presence or absence of a blocker of the autophagic flux, enables for the discrimination of autophagy inducers from inhibitors, since both classes can trigger an accumulation of autophagosomes.