The skin is richly supplied with a vascular network, largely exceeding its nutritive requirements. Skin microcirculation is a fundamental actor involved in thermoregulation, wound healing, immune reactions and the control of blood pressure. It is also a major actor of skin redness, rosacea and discreet erythema.

We offer many in vitro models to evaluate :

- Angiogenesis and neovascularization stimulation (HUVEC / NHDF co-culture)
- Endothelial cells signaling (AMPc and calcium measurement)
- Protection of endothelial cells against stress (…)

Evaluation CGRP receptors antagonists

A functional assay assessing antagonistic properties of compounds on endogenous CGRP receptors, activated with the potent vasodilator CGRP (Calcitonin Gene-Related Peptide). Primary Human Dermal Microvascular Endothelial Cells were seeded in 96-well plates. Since CGRP receptors are Gs-coupled GPCRs, cAMP accumulation was quantitated, by HTRF. The dose-dependent aCGRP-induced cAMP production was rightward-shifted by the reference antagonist CGRP 8-37, indicating inhibition.

Evaluation of angiogenesis (NHDF/HUVEC)

Angiogenesis processes (tube formation and tube length quantification) can be monitored in a 96-well plate format. For example a co-culture of normal human dermal fibroblast (NHDF) and GFP-labeled human umbilical vein endothelial cells (HUVEC) is performed (Essen bioscience kit). Tube length is kinetically monitored for 11 days in-vitro using VEGF as pro-angiogenic control and Axitinib as anti-angiogenic reference.