IN VITRO TOXICITY TESTING

We offer several in-vitro tests to assess the safety of your ingredients, providing an effective and ever-improving alternative to animal testing. In some areas, such as cosmetics destined for the EU, this approach replaces animal testing completely.

These tests are also compatible with the ISO 10993 guidelines which entail a series of standards for evaluating the biocompatibility of medical devices

Our in vitro models can evaluate :

- Cytotoxicity in fibroblast cells (3T3, L929)
- Skin irritation - Episkin (OECD 439)
- Occular irritation HET-CAM

Neutral red uptake in 3T3 fibroblastic cells


The cytotoxicity of the test compound to BALB/c 3T3 mouse fibroblasts is assessed by Neutral Red Uptake. After Neutral Red incorporation in living cells, the optic density is measured using a colorimeter. This test is compatible with the ISO 10993 guidelines.

MTT assay in L929 cells fibroblasts


The cytotoxicity of the test compound to L929 fibroblasts is assessed by MTT uptake. After MTT reduction in formazan in living cells, the optic density is measured using a colorimeter. This test is compatible with the ISO 10993 guidelines.

in Vitro Skin Irritation - OECD 439

This uses reconstructed human epidermis (RhE) Episkin model, which closely mimics the upper parts of the human skin. Cell viability is measured by enzymatic conversion of the vital dye MTT into a blue formazan salt that is quantitatively measured after extraction from tissues. Irritant test substances are identified by their ability to decrease cell viability below defined threshold levels (below or equal to 50%).

This test is compatible with the ISO 10993 and OECD 439 guidelines.

Occular irritation HET-CAM (Hen's Egg Test ChlorioAllantoic Membrane)


In the HET-CAM test chemicals are placed in direct contact with the chorioallantoic membrane of the chicken's egg. The occurrence of vascular lysis, coagulation and hemorrhage in response to a compound is the basis for employing this technique as an indication of the likelihood of a chemical to damage mucous membranes (especially the eye) in vivo.