ANTI-AGING & FIRMING ACTIVITY

Skin aging is a complex biological process influenced by a combination of endogenous and exogenous / extrinsic factors. A lot of skin anti-aging strategies attempted to reverse the dermal and epidermal signs of ageing by blocking exposome damages or boosting dermal / epidermal regeneration. PORSOLT offers in vitro models to evaluate products or active ingredients :

- Cell proliferation, migration (NHDF, HaCaT)
- Protection against oxidative stress (NHEK, HaCaT) 
- Collagen synthesis (NHDF)
- GSH / ROS and proteasome (20S) measurements
- Senscence measurement

We can also stain Reconstructed human epidermis or ex-vivo human skin explants for :

- Morphology of skin tissue such as epidermal thickness, morphology of epidermis layers
- Proliferation (KI-67)

Senescence measurement for anti-aging claims


Senescence can be induced by using low concentrations and short exposure to hydrogen peroxide. Cells exhibit senescent-morphological features, and senescence-associated b-galactosidase positivity (4 times increase – left graph) which can be correlated with a diminution in their ability to invade the wound in wound healing assay (left graph). Anti-aging ingredients can be challenged against this model to show i) decrease in induced-senescence  ii) stimulation of senescent-keratinocyte (e.g. EGF in right graph).

Collagen synthesis in NHDF


Normal Human Dermal Fibroblasts (NHDF) were seeded in 96-well plates. After treatment with reference compound (Ascorbic acid at 300 µM and TGF-beta at 30 ng/mL for 72h), the total collagen at the level of the cell layer was quantified with Sirius Red staining and absorbance measurement.

TEST SHEET - ANTI-AGING & FIRMING ACTIVITY